Spectral correction for attenuating retroactivity in synthetic biology networks
Journal Title: International Journal of engineering Research and Applications - Year 2017, Vol 7, Issue 8
Abstract
The reliability of synthetic biology systems is challenged by the undesired retroactive signals transmitted by the downstream modules to the upstream modules. These retroactive signals modify the input and output signals of the system, limiting the predictability of the system. This is viewed as similar to the loading effect on electrical, mechanical or hydraulic systems and is being studied using the typical engineering strategies. Here, we propose spectral analysis as a tool to explore and propose methods to attenuate this phenomenon. We select an existing system constituted by an input protein, which phosphorylates another protein to form the output, which further binds to another module in the downstream. The input signal with downstream connection is spectrally decomposed to four key components. We report how the dynamics of the system varies for each of the four components and its combinations. Our results show that the removal of the first harmonic component of the retroactive input signal can attenuate the retroactive effect. The biological significance and wet lab protocols for achieving this spectral correction is to be investigated further.
Authors and Affiliations
Silpa Bhaskaran, Achuthsankar S. Nair
Keywords
Related Articles
- EP ID EP391954
- DOI 10.9790/9622-0708025965.
- Views 111
- Downloads 0
Use of Bitumen Emulsion for Flexible Road Construction
In the present study bitumen is replaced by bitumen emulsion for the construction of flexible pavement. The conventional method of road construction involves the burning of bitumen which produces toxic gases which degrad...
Impression of (PEO+KBrO3+Plasticizer) Polymer Electrolyte as Carbon Monoxide Gas Sensor
Solid state polymer electrolytes have been prepared by with poly (ethylene oxide) (PEO), KBrO3 salt and plasticizer (Dimethyle Formamide [DMF]) by using solution – casting technique. These polymer electrolytes have been...
Study theImpact ofEarthquake load on Behavior of I Shape RC Shear Wallsat the Centerof Structure
The usefulness of shear walls in the structural planning of multistory buildings has long been recognized .Shear walls are located on each level of the structure, to form an effective box structure, equal length shear wa...
Biological Sequence Alignment - A Review
Bioinformatics is an emerging interdisciplinary research area that deals with the computational management and analysis of biological information. Genomics is the most important domain in bioinformatics which compares ge...
Process Parameter Optimization for Tensile Strength for Plasma Arc Welding of SS304
The Joining of the Austenitic Stainless Steels is often used in the pressure vessels, chemicals and processing industries. The mechanical properties at the weld bead should also fulfill requirement of applicable code. Se...