SPECTROPHOTOMETRIC DETERMINATION AND VALIDATION OF GLIMEPIRIDE IN PURE AND TABLET DOSAGE FORMS THROUGH ION-PAIR COMPLEX FORMATION USING BROMOCRESOL GREEN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 6
Abstract
Objective: A simple, direct and accurate spectrophotometric method has been developed for the determination of glimepiride (GLM) in pure and pharmaceutical formulations by complex formation with bromocresol green (BCG).Methods: The method involves the formation of a yellow ion-pair complex between bromocresol green reagent with glimepiride (C24H34N4O5S); after reacted it with Na2CO3 to give C24H33N4H+O5NaS in chloroform at pH≤3.8.Results: The formed complex was measured at lmax 416 nm against the reagent blank prepared in the same manner. Variables were studied in order to optimize the reaction conditions. Beer’s law was obeyed in the concentration range of 0.981-9.812 μg/ml in the present of 1x10-4 mol/l of (BCG) and 9.812-58.874 μg/ml in the present of 1x10-3 mol/l of (BCG) with good correlation coefficient (R2= 0.9992 and R2= 0.9997, respectively). The relative standard deviation did not exceed 3.0%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.088 and 0.29 μg/ml, respectively. The proposed method was validated for specificity, linearity, precision and accuracy, repeatability, sensitivity (LOD and LOQ), and robustness with average recovers 98.9 to 102.4%.Conclusion: The developed method is applicable for the determination of glimepiride in pure and different dosage forms with average assay of marketed formulations 97.8 to 102.4% and the results are in good agreement with those obtained by the RP-HPLC reference method.Keywords: Direct spectrophotometric method, Glimepiride, Bromocresol green, Ion-pair complex
Authors and Affiliations
Abdul Aziz Ramadan, Hasna Mandil, Souad Zeino
Keywords
Related Articles
- EP ID EP576337
- DOI -
- Views 63
- Downloads 0
A PHARMACOLOGICAL COMPREHENSIVE REVIEW ON ‘RASSBHARY’ PHYSALIS ANGULATA (L.)
The present review article reveals the importance of species Physalis angulata (L.) of the genus Physalis (L.) distributed worldwide including India. Physalis species are perennial, erect and variously having toothed or...
ASSESSMENT OF THE NUTRITIONAL BEHAVIOUR AMONG COLLEGE STUDENTS-A SURVEY
Objective: To assess the nutritional behaviour among college students.Methods: A prospective observational survey was conducted randomly among college students in Guntur. A self-administered data collection form was desi...
BETULIN-3, 28-DIPHOSPHATE SALT COMPLEXES WITH AMINES AND THEIR ANTIOXIDANT ACTIVITY
Objective: Studies of composition, stability and antioxidant properties of the betulin-3, 28-diphosphate complexes with dopamine and trisamine.Methods: The betulin-3, 28-diphosphate (BDP) interaction with amines in a wat...
METHOD DEVELOPMENT FOR QUANTIFICATION OF OXIDATION COMPLEXES OF NADOLOL AND RESVERATROL BY VISIBLE SPECTROPHOTOMETRY
Objective: The present work is a report on two colorimetric methods based on oxidation and complexation of nadolol and resveratrol with ferric chloride and 2, 2'-bipyridyl.Methods: The methods were developed for Perkin E...
BENEFICIAL EFFECTS OF ETHANOLIC LEAF EXTRACT OF CORIANDRUM SATIVUM ON METANIL YELLOW INDUCED ALTERATION INACTIVITY OF CATALASE AND LEVEL OF LIPID PEROXIDATION IN HERCINE CARDIAC TISSUE IN VITRO
Objective: The primary aim of the study was to find out a suitable effective dose of metanil yellow that can cause alterations of lipid peroxidation and catalase activity in the heart in vitro and to determine the effect...