SPECTROPHOTOMETRIC DETERMINATION AND VALIDATION OF GLIMEPIRIDE IN PURE AND TABLET DOSAGE FORMS THROUGH ION-PAIR COMPLEX FORMATION USING BROMOCRESOL GREEN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 6
Abstract
Objective: A simple, direct and accurate spectrophotometric method has been developed for the determination of glimepiride (GLM) in pure and pharmaceutical formulations by complex formation with bromocresol green (BCG).Methods: The method involves the formation of a yellow ion-pair complex between bromocresol green reagent with glimepiride (C24H34N4O5S); after reacted it with Na2CO3 to give C24H33N4H+O5NaS in chloroform at pH≤3.8.Results: The formed complex was measured at lmax 416 nm against the reagent blank prepared in the same manner. Variables were studied in order to optimize the reaction conditions. Beer’s law was obeyed in the concentration range of 0.981-9.812 μg/ml in the present of 1x10-4 mol/l of (BCG) and 9.812-58.874 μg/ml in the present of 1x10-3 mol/l of (BCG) with good correlation coefficient (R2= 0.9992 and R2= 0.9997, respectively). The relative standard deviation did not exceed 3.0%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.088 and 0.29 μg/ml, respectively. The proposed method was validated for specificity, linearity, precision and accuracy, repeatability, sensitivity (LOD and LOQ), and robustness with average recovers 98.9 to 102.4%.Conclusion: The developed method is applicable for the determination of glimepiride in pure and different dosage forms with average assay of marketed formulations 97.8 to 102.4% and the results are in good agreement with those obtained by the RP-HPLC reference method.Keywords: Direct spectrophotometric method, Glimepiride, Bromocresol green, Ion-pair complex
Authors and Affiliations
Abdul Aziz Ramadan, Hasna Mandil, Souad Zeino
Keywords
Related Articles
- EP ID EP576337
- DOI -
- Views 59
- Downloads 0
UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND ACECLOFENAC IN BULK AND TABLET DOSAGE FORM
Objective: To develop a simple, economic and validated UV spectrophotometric method for the simultaneous estimation of aceclofenac and tramadol hydrochloride in bulk and tablet dosage form.Methods: The UV spectrophotomet...
FACILE SYNTHESIS, SPECTRAL STUDIES, DFT CALCULATIONS AND BIOLOGICAL ACTIVITIES OF NOVEL NI (II), CU (II), AND PD (II) COMPLEXES OF THIADIAZOLE ANALOGS
Objective: A facile synthesis of some novel Schiff base derivatives of 2-substituted-5-amino-thiadiazoles along with their Ni (II), Cu (II), and Pd (II) complexes were achieved by sonication and the conventional method....
ANTIGENICITY OF HYDROLYZED RICE FORMULA IN ANIMAL MODEL (BALB/C MICE)
Objective: The hydrolyzed formulas of bovine proteins used in the cow's milk allergy treatment contain peptides which can preserve their allergenicity. These last years, a new preparation with hydrolyzed rice proteins wa...
Evaluation of appropriateness of prescription and polypharmacy in the geriatric population: a cross sectional study at a comprehensive geriatric clinic in a tertiary care hospital
Objective: To assess the prescription quality in a comprehensive geriatric clinic and to determine the frequency of inappropriate prescription and polypharmacy.Methods: Both males and female patients above the age of 60...
NON PITTING TYPE PEDAL EDEMA WITH LITHIUM: A CASE REPORT
Objective: To report a case of lithium induced bilateral nonpitting pedal edema.Methods: The clinical data of a bipolar affective disorder patient with current episode of mania and psychotic symptoms who experienced bila...