Spectrophotometric Determination of Sun Protection Factor and Antioxidant Potential of an Herbal Mixture

Journal Title: Biotechnology Journal International - Year 2016, Vol 10, Issue 3

Abstract

Objectives: Present research work deals with the determination of hydroxyl radical scavenging potential and Sun Protection Factor of herbal mixture which was prepared by the phytochemical composition of different herbal extracts. Materials and Methods: The herbal mixture was prepared by the composition of important herbal plant extracts such as; 50% ethanolic extract of Berberis aristata root, 30% ethanolic extract of Ficus benghalensis bark, ethanolic extract of Asparagus racemosus root, aqueous extract of Asparagus racemosus root, 30% methanolic extract of Butea monosperma flowers, gel extract of Aloe vera, 80% ethanolic extract of Terminalia arjuna bark, 80% ethanolic extract of Cyperus rotundus root, 80% ethanolic extract of Rubia cordifolia root and 50% methanolic extract of Hibiscus-rosa-sinensis flowers, which was further proceed for hydroxyl radical scavenging activity and Sun Protection Factor determination at different concentrations viz;0.5%, 1%, 5% and 10%. Results: IC50 values of Ascorbic acid was found to be 52.93±2.64% (Inhibition TBARS) at the concentration of 18.00 µg/ml and herbal mixture were 51.58±1.27% (Inhibition TBARS) observed at the concentration of 9.80 µg/ml respectively. The SPF values for different concentrations of herbal mixture were in between 2.14±0.15SPF to 12.97±0.07SPF. The results showed that 10% concentrated of herbal mixture has high SPF value of 12.97±0.07SPF which may be attributed due to the presence of active components. Conclusion: Herbs and herbal preparations have high potential due to their antioxidant activity, primarily. The bioactive compounds such as flavonoids, phenolic acids, saponins etc. of this prepared herbal mixture may able to reduce skin damages which are caused due to long time exposure of skin in sun rays specially UVA and UVB rays. The proposed spectrophotometric method is simple and rapid for the in vitro determination of SPF values of sunscreens emulsions.

Authors and Affiliations

Varsha Sharma, Madhulika Singh

Keywords

Related Articles

Antimicrobial Effect Induced by Fresh Ginger Root Extracts in Broilers

Poultry is challenged by microbial infection owing to the restrictions in the use of synthetic antibiotic growth promoters. This study investigated the use of ginger, Zingiber officinale Roscoe (family Zingiberaceae) for...

Cell Proliferation Using Broccoli Leaf Cutting in vitro Culture: Its Biochemical and Antioxidant Properties

Significance of the study: Tissue or cell culture keeps a significant role in micro-propagation in the plant production industry. Nowadays it has been successfully performed from many species in plant. Millions of explan...

Sequence Homology Studies of Phospholipase A2-like Gene from Bloodstream form of Trypanosoma brucei

Aim: This work focused on the sequence homology studies of the enzyme, phospholipase A2 (PLA2), in Trypanosoma brucei obtained from the blood of bull in Federe, Plateau State, Nigeria, West Africa Place and Duration o...

Optimization of Polyphenols Extraction Method from Kola Nuts (Cola nitida Vent. Schott & Endl.) Using Experimental Design

Aims: The aim of this study is to optimize extraction process of phenolics compounds from kola nuts by using experimental design. Study Design: Kola nuts were collected in October 2014-February 2015 in south of Côte d’Iv...

Partial Purification of Cellulase Enzyme from Fusarioum oxysporium by Using Egg White Matrix

Aim: Protein matrix possesses a capacity to bind organic and inorganic matter. Egg white matrix as an adsorbent was utilized for partial purification of cellulase enzyme in detailed batch study. Methodology: Effect of pH...

Download PDF file
  • EP ID EP237693
  • DOI 10.9734/BBJ/2016/21434
  • Views 132
  • Downloads 0

How To Cite

Varsha Sharma, Madhulika Singh (2016). Spectrophotometric Determination of Sun Protection Factor and Antioxidant Potential of an Herbal Mixture. Biotechnology Journal International, 10(3), 1-8. https://europub.co.uk/articles/-A-237693