SPECTROPHOTOMETRIC DETERMINATION OF VITAMIN E VIA FORMATION OF GOLD COMPLEX
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 7
Abstract
Two different methods were used for the determination of vitamin E (Tocopherol) via complex formation with gold ions in solutions using UV - VIS spectrophotometry and flame atomic absorption spectrophotometry (AAS). The absorption maxima of vitamin E – gold complex was found to be at (535 nm) and this wavelengths was selected for the analysis of vitamin E as standard and formulated samples. The optimum conditions were investigated through the study of different parameter such as pH, temperature, concentration, time and complex formula. Stability constants for the complex was (5.91 x 105). The purposed method obeyed Beer-Lambert's Law in the concentration range of (2 - 40 μg/mL), (1 – 22 μg/mL) with R2 values of (0.9991, 0.9992) for UV-VIS and atomic spectroscopic methods respectively. The relative standard deviation (1.95, 1.59), detection limits (0.18, 0.044) and recoveries (96.86 and 101.66%) for the first and second method respectively. The methods were applied for the estimation of the active gradient of the vitamin E in different samples of formulated dosage. The accuracy of method was validated by mean percentage recovery which was found to be in the acceptable range.
Authors and Affiliations
Dr. Ahmed Mahdi Saeed
Keywords
Related Articles
- EP ID EP628832
- DOI -
- Views 147
- Downloads 0
NUTRITIONAL POTENTIAL, MINERAL COMPOSITION AND ANTIOXIDANT ACTIVITY SQUASH (CUCURBITA PEPO L.) FRUITS GROWN IN EGYPT.
The nutritional potential of fruits, fruit pulp and seeds of Squash (Cucurbita pepo L.) collected from Giza, Egypt was evaluated for their proximate values and mineral composition. In addition to; the total phenolic and...
IN-HOSPITAL REBLEEDING FOLLOWING PERIMESENCEPHALIC SUBARACHNOID HEMORRHAGE
Perimesencephalic subarachnoid hemorrhage is a variant of subarachnoid hemorrhage of unknown etiology and generally good prognosis. In-hospital rebleeding is an extremely rare but potentially lethal complication of perim...
SUCCESSFUL MANAGEMENT OF NON CARDIAC SURGERY IN A PATIENT WITH UNREVASCULARISED TRIPLE VESSEL DISEASE
Sir/ Madam, Patient with unrevascularized severe coronary artery disease (CAD) poses a great challenge to the anaesthesiologist and can lead to adverse perioperative cardiac outcomes if not properly managed.
A NOVEL CLASSIFICATION FOR FINGER FRICTION RIDGES (DERMATOGLYPHIC PATTERNS)
Since 200 BC till date, researchers and investigators have studied the fingerprint patterns for extensive purpose using different classifications that have proven useful in personal identification, hereditary, genetic pr...
INVESTIGATION OF THE ANTIMICROBIAL ACTIVITIES OF Phyllanthus urinaria LEAVES BEFORE AND AFTER COMBINED WITH Pandanus tectorius FRUIT EXTRACTS
The study was to investigate the antimicrobial activities of Phyllanthus urinaria leaves extracts before and after combined with Pandanus tectorius fruit extracts. This was accomplished by examining the influence of the...