SPECTROPHOTOMETRIC DETERMINATION OF VITAMIN E VIA FORMATION OF GOLD COMPLEX
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 7
Abstract
Two different methods were used for the determination of vitamin E (Tocopherol) via complex formation with gold ions in solutions using UV - VIS spectrophotometry and flame atomic absorption spectrophotometry (AAS). The absorption maxima of vitamin E – gold complex was found to be at (535 nm) and this wavelengths was selected for the analysis of vitamin E as standard and formulated samples. The optimum conditions were investigated through the study of different parameter such as pH, temperature, concentration, time and complex formula. Stability constants for the complex was (5.91 x 105). The purposed method obeyed Beer-Lambert's Law in the concentration range of (2 - 40 μg/mL), (1 – 22 μg/mL) with R2 values of (0.9991, 0.9992) for UV-VIS and atomic spectroscopic methods respectively. The relative standard deviation (1.95, 1.59), detection limits (0.18, 0.044) and recoveries (96.86 and 101.66%) for the first and second method respectively. The methods were applied for the estimation of the active gradient of the vitamin E in different samples of formulated dosage. The accuracy of method was validated by mean percentage recovery which was found to be in the acceptable range.
Authors and Affiliations
Dr. Ahmed Mahdi Saeed
Keywords
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