SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF TOTAL ALKALOIDS IN THE TINOSPORA CORDIFOLIA M. AND ITS HERBAL FORMULATIONS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: A simple spectrophotometric method was developed for the estimation of total alkaloids in Tinospora cordifolia M. and its formulations.Methods: The method based on the formation of yellow colored complex by reaction between bromocresol green (BCG) and alkaloids in medicinal plants. A yellow colored complex forms is easily extractable by chloroform at pH 4.7.Results: The method was linear in concentration range of 2-10 μg/ml with λmax at 415 nm. The LOD and LOQ were found to be 0.215 and 0.652 respectively. The intra-day and inter-day precision and accuracy were within the acceptable criteria (relative standard deviation<2.0% and accuracy within 100±2%). Conclusion: The developed method is simple, precise and accurate and can be adopted for the routine quality control and standardization of plant materials containing alkaloids and its pharmaceutical products.Â
Authors and Affiliations
Rajendra K. Patel, Jigar B. Patel, Priti D. Trivedi
Keywords
Related Articles
- EP ID EP578284
- DOI -
- Views 82
- Downloads 0
EXPLORATION OF IN VITRO ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF MOMORDICA CYMBALARIA FENZL LEAVES EXTRACTSEXPLORATION OF IN VITRO ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF MOMORDICA CYMBALARIA FENZL LEAVES EXTRACTS
Objective: The target of this current research work sought to analyze the antioxidant and antimicrobial activities of ethyl acetate and hydro-alcoholic extracts of Momordica cymbalaria leaves through various in vitro ass...
EVALUATION OF DIURETIC AND ANTIUROLITHIATIC PROPERTIES OF ETHANOLIC EXTRACT OF SIDA ACUTA BURM. F. IN WISTAR ALBINO RATS
Objective: To evaluate the diuretic and antiurolithiatic properties of ethanolic extract of Sida acuta Burm. f. in albino rats.Methods: Three different doses of Sida acuta (100 mg/kg, 200 mg/kg, 400 mg/kg) were tested fo...
NOSOCOMIAL INFECTION BY NON-FERMENTING GRAM NEGATIVE BACILLI IN TERTIARY CARE HOSPITAL: SCREENING AND CURE
Objective: The purpose of the present study was to identify the Non-Fermenting Gram Negative Bacteria from the tertiary care hospital.Methods: The conventional method was used i.e. Antibiotic susceptibility test which wa...
THE CONTRIBUTION OF CHOLINERGIC NEUROCIRCUITS OVER THE MODULATION OF DOPAMINE ACTIVITY IN THE STRIATUM
The dopaminergic neurons and cholinergic interneurons of the striatum play key roles in motivational and sensorimotor processing by the basal ganglia. The striatal dopaminergic transmission in the forebrain is modulated...
OBSERVATIONAL STUDY ON POISON CASES IN A TERTIARY CARE HOSPITAL
Objective: To evaluate the prevalence, pattern, and cause of poisoning. To characterize the poisoning cases admitted in a tertiary care hospital; followed by the outcome and to observe the antidote given for the poison c...