SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF TOTAL ALKALOIDS IN THE TINOSPORA CORDIFOLIA M. AND ITS HERBAL FORMULATIONS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: A simple spectrophotometric method was developed for the estimation of total alkaloids in Tinospora cordifolia M. and its formulations.Methods: The method based on the formation of yellow colored complex by reaction between bromocresol green (BCG) and alkaloids in medicinal plants. A yellow colored complex forms is easily extractable by chloroform at pH 4.7.Results: The method was linear in concentration range of 2-10 μg/ml with λmax at 415 nm. The LOD and LOQ were found to be 0.215 and 0.652 respectively. The intra-day and inter-day precision and accuracy were within the acceptable criteria (relative standard deviation<2.0% and accuracy within 100±2%). Conclusion: The developed method is simple, precise and accurate and can be adopted for the routine quality control and standardization of plant materials containing alkaloids and its pharmaceutical products.Â
Authors and Affiliations
Rajendra K. Patel, Jigar B. Patel, Priti D. Trivedi
Keywords
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