Sperm respiration intensity and survival of spermatozoa with taurine addition in diluted bull ejaculates
Journal Title: Біологія тварин - Year 2014, Vol 16, Issue 3
Abstract
Semen respiration intensity and spermatozoa survival by using diluents with different taurine concentration in bull ejaculates has been studied. Intensity of oxygen consumption by semen was determined polarographycally with Clark electrode usage (at temperature 38.5 °С), spermatozoa survival — by storage with dilution (at temperature 0–4 °С) till linear forward motion is stopped (h). To diluted semen, with phosphate-saline buffer and synthetic mediums (lactose-yolk-glycerin diluent and “Andromed”) taurine in concentrations 0.175; 0.35; 0.525 and 0.7 mmol/l was added. In our studies we determined correlation between semen respiration rate and spermatozoa survival, with using diluents and at action of taurine in different concentrations. With taurine concentration increasing, sperm respiration intensity decreased. The biggest decrease of indexes was after diluting semen with phosphate-saline buffer and the smallest — after mixing with Andromed. By diluting semen with phosphate-saline buffer, survival increases with taurine concentration. When bull ejaculates were diluted by lactose-yolk-glycerin diluents spermatozoa survival dependence had a maximal index with addition 0.35 mmol/l, though changes haven’t reached first degree of probability. In case of sperm dilution by Andromed, spermatozoa survival decreased with taurine concentration increase, and while adding 0.7 mmol/l, was lower on 18.2 % comparing to control. Possible reason for positive taurine impact on spermatozoa survival is ability to maintain transmembrane mitochondrial potential and influence on substrate utilization, its antioxidant properties, in case of negative effect — ability to cotransport with Na+, that decreases spermatozoa motility.
Authors and Affiliations
R. D. Ostapiv, V. V. Manko, I. M. Yaremchuk, D. D. Ostapiv
Keywords
Related Articles
- EP ID EP366126
- DOI 10.15407/animbiol16.03.110
- Views 86
- Downloads 0
Histological structure of separate internal organs of piglets at application of probiotic feed additive
The aim of the test was to study the influence of probiotic feed additive in doses recommended by the producer and duration of application on the histological structure of separate internal organs of piglets. The product...
Expression of imunoreceptor proteins in plasmolemma of enterocytes in formation of colostric immunity in newborn calves
Indexes of content of proteins with molecular masses of 37, 40 and 43 kDa in plasmolemma of small intestine enterocytes in newborn calves in dynamics from the birth till the age of 24 hours were investigated. The researc...
Biochemical processes in the cows and biological value of milk under influence of chromium, selenium, cobalt and zinc citrates
The effect of citrates of trace elements produced by nanotechnology on biochemical processes in the lactating cows, average milk yield and performance and its biological value has been studied. The experiment was carried...
Chemical composition and nutritional values of feed resources for deer
Increasing of wild animals populations and saving of endangered species require proper providing of nutrient needs. In general, the available feed resources provide the feed needs of wild herbivores, but the ratio of the...
Analysis of the dynamics of antibody synthesis in horse serum after vaccination with UA-07 “Antravak” against anthrax of Bacillus anthracis strain
The results of research to determine the level of antibody titer of anthrax in horses of all ages after a vaccine against anthrax strain of animals Bacillus anthracis UA–07 “Antravak” are presented in the paper. For the...