Sperm respiration intensity and survival of spermatozoa with taurine addition in diluted bull ejaculates
Journal Title: Біологія тварин - Year 2014, Vol 16, Issue 3
Abstract
Semen respiration intensity and spermatozoa survival by using diluents with different taurine concentration in bull ejaculates has been studied. Intensity of oxygen consumption by semen was determined polarographycally with Clark electrode usage (at temperature 38.5 °С), spermatozoa survival — by storage with dilution (at temperature 0–4 °С) till linear forward motion is stopped (h). To diluted semen, with phosphate-saline buffer and synthetic mediums (lactose-yolk-glycerin diluent and “Andromed”) taurine in concentrations 0.175; 0.35; 0.525 and 0.7 mmol/l was added. In our studies we determined correlation between semen respiration rate and spermatozoa survival, with using diluents and at action of taurine in different concentrations. With taurine concentration increasing, sperm respiration intensity decreased. The biggest decrease of indexes was after diluting semen with phosphate-saline buffer and the smallest — after mixing with Andromed. By diluting semen with phosphate-saline buffer, survival increases with taurine concentration. When bull ejaculates were diluted by lactose-yolk-glycerin diluents spermatozoa survival dependence had a maximal index with addition 0.35 mmol/l, though changes haven’t reached first degree of probability. In case of sperm dilution by Andromed, spermatozoa survival decreased with taurine concentration increase, and while adding 0.7 mmol/l, was lower on 18.2 % comparing to control. Possible reason for positive taurine impact on spermatozoa survival is ability to maintain transmembrane mitochondrial potential and influence on substrate utilization, its antioxidant properties, in case of negative effect — ability to cotransport with Na+, that decreases spermatozoa motility.
Authors and Affiliations
R. D. Ostapiv, V. V. Manko, I. M. Yaremchuk, D. D. Ostapiv
Keywords
Related Articles
- EP ID EP366126
- DOI 10.15407/animbiol16.03.110
- Views 95
- Downloads 0
The indices of antioxidant defense system in piglets under ferum citrate action
The data about the indices of antioxidant defense system in piglets after intramuscular injection the different doses of ferum citrate (100 ml of solution contains 0.4 mg Fe) for the ferum deficiency anemia prophylaxis w...
Effect of glucose and partial dehydration on resistance of mammalian erythrocytes to hypertonic shock
The paper is devoted to the studying the resistance of human, rabbit and rat erythrocytes to the effect of hypertonic shock when changing an initial status of cells modified with glucose and 0.4 mol/l NaCl. The hemolysis...
Histological structure of separate internal organs of piglets at application of probiotic feed additive
The aim of the test was to study the influence of probiotic feed additive in doses recommended by the producer and duration of application on the histological structure of separate internal organs of piglets. The product...
Influence of typological features of autonomous tone on morphometric parameters of goblet cells of chickens’ intestinal mucosa
In adult chickens cross “Iza-Braun”, which were grown in conditions of poultry farms and were selected on the basis of analogues, there are two types of autonomous tone: sympathotonic (CT) — 16 specimen and sympatho-norm...
Effects of exogenous cholesterol and amidated cellulose on fat and sterols concentration in faeces
The increased conversion of cholesterol to bile acids in the liver compensates for the loss of bile acids in faeces. This leads to lower cholesterol levels in the blood. An alternative to bile acid sequestrants is sorben...