STABILITY INDICATING GREEN ANALYTICAL GAS CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF DIPHENHYDRAMINE IN PURE AND MARKETED FORMULATIONS
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 9
Abstract
The current work defines development of a simple, rapid and precise method based on gas chromatography coupled with Flame Ionization Detector (GC-FID) for the quantitative estimation of diphenhydramine (DPH) present in the marketed formulations following the principles of green analytical chemistry. The analysis was performed using Zebron Phase: ZB-DRUG-1 column with Nitrogen as a carrier gas having a flow rate of 1 ml/min. The injection volume was 1 µL and with the split ratio of 1:10. The retention time of the drug was found to be 4.5 minutes. The developed method was validated according to the ICH guidelines, proving the method to be sensitive towards the analyte with accuracy (mean recovery = 98%), linearity in the range of 2-10 µg/ml (r2 0.993), precision (intraday = 1.118% RSD, interday = 1.16 % RSD), LOD 0.2 µg/ml and LOQ 0.5 µg/ml. The degradation study showed that the drug remains stable in acid, base, neutral, oxidation, and photolytic method. The developed method can be effectively employed for the day to day analysis of DPH in pure and marketed formulations by industries and not so well equipped laboratories, as the method employs direct analysis of DPH without any derivatization technique by gas chromatography with simple flame ionization detection.
Authors and Affiliations
B. M. Gurupadayya et al.
Keywords
Related Articles
- EP ID EP627492
- DOI 10.13040/IJPSR.0975-8232.10(9).4280-86
- Views 48
- Downloads 0
CORRELATION OF ANKLE/BRACHIAL INDEX AND DIABETIC RETINOPATHY IN TYPE 2 DIABETIC PATIENTS’
This study aims to correlate the ankle/brachial index (ABI) with the severity of diabetic retinopathy in type 2 diabetic patients. Material and Methods: A hospital-based prospective study of 100 types 2 diabetic patients...
SYNTHESIS AND CHARACTERIZATION OF 2-(PYRIDIN-2-YL)GUANIDINE DERIVATIVES AND THEIR METAL COMPLEXES AS POTENTIAL ANTIBACTERIAL AGENTS USING PHOSPHORYL CHLORIDE
Guanidines were prepared by the condensation reaction of primary amine (-NH2) with urea derivatives (R2NCONR2) in the presence of POCl3. In this research guanidine-pyridine, hybrid derivatives were synthesized by substit...
SYNTHESIS AND ANTIBACTERIAL SCREENING OF FIVE NEW AZOMETHINE DERIVATIVES OF 2-AMINO-2-METHYL-1-PROPANOL
In this present investigation author reporting the anti-bacterial activity of five new azo methines, namely 2-((1-hydroxy-2- methyl propan-2-ylimino) methyl)-6-methoxyphenol (AZM1), 4-((1-hydroxy-2- methyl propan-2-ylimi...
IN-VITRO COMPARATIVE ANTICANCER ACTIVITY STUDY OF METHANOLIC EXTRACT OF TRADITIONALLY USED MEDICINAL PLANT OF MIZORAM
Objective: The present study explores the anticancer potentially of plant sample based on the evidence from the ethnomedicinal practice of the plant. The main objective was to find out the in-vitro comparative anticancer...
PROTECTIVE EFFECT OF PERILLYL ALCOHOL (POH) A MONOTERPENE: ON HIGH FAT DIET INDUCED HYPERLIPIDEMIA IN ALBINO WISTAR RATS A PRELIMINARY STUDY
To assess the effect of Perillyl alcohol (POH) on the lipid profile of high-fat-diet-induced hyperlipidemia in white male albino Wistar rats, the hyperlipidemia was induced by feeding cholesterol-rich high-fat diet for 4...