STABILITY INDICATING HPTLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF AMBROXOL HYDROCHLORIDE AND CETIRIZINE DIHYDROCHLORIDE IN COMBINE TABLET DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 3
Abstract
A new simple, sensitive and precise high performance thin layer chromatographic method has been developed for estimation of Ambroxol hydrochloride and Cetirizine dihydrochloride simultaneously from a combined Solid dosage form.In this method pre coated silica gel 60 GF 254 TLC plate was used as stationary phase and the chromatogram was developed using CCl4: Chloroform: Methanol (3:5:2v/v/v) as mobile phase. Ambroxol hydrochloride and Cetirizine dihydrochloride showed Rf values 0.74 ± 0.2, 0.47 ± 0.2 respectively. The plate was scanned and quantified at 230 nm using Camag TLC Scanner. The linear concentration 240-720ng/band and 20-60 ng/band for Ambroxol hydrochloride and Cetirizine dihydrochloride respectively. The percentage recovery for Ambroxol hydrochloride and Cetirizine dihydrochloride was found to be 100.08 % , 101.21%. As the method could effectively separate the drug from its degradation product it can be employed as stability indicating one. The method is validated as per ICH guidelines.
Authors and Affiliations
Advaita Patel , Sanket Patel , Deepa Patel , Bhavesh Patel , Madhabhai Patel
Keywords
Related Articles
- EP ID EP145450
- DOI -
- Views 97
- Downloads 0
RecA: A NOVEL TARGET FOR DRUG DELIVERY
The RecA protein is a recombinase functioning in recombinational DNA repair in bacteria. RecA is regulated at many levels. The expression of the recA gene is regulated within the SOS response. The activity of the RecA pr...
PREDICTORS OF FETO–MATERNAL MORBIDITY IN PREGNANCY WITH CHRONIC HYPERTENSION
Chronic hypertension affects 0.5-5% of pregnant women. Ninety percent is due to essential hypertension and rest due to secondary causes. It is associated with both maternal and fetal complications. This is a prospective...
STUDY OF ANTIULCER ACTIVITY OF ROOTS OF ALANGIUM SALVIFOLIUM LINN. IN PYLORUS LIGATED RATS
Different extracts of Alangium salvifolium Linn. roots were tested for its antiulcer activity in aspirin induced and pylorus ligated rats model. The effect was assessed by parameters like Total acidity(TA),Free acidity(F...
FORMULATION AND EVALUATION OF DISPERSIBLE TABLETS OF AMOXICILLIN TRIHYDRATE AND DICLOXACILLIN SODIUM
In the present work attempts were made to prepare dispersible tablets of Amoxicillin trihydrate and Dicloxacillin sodium by direct compression technique to enhance patient compliance. The three superdisintegrents used in...
EVALUATION OF ANTI-NEPHROLITHIATIC ACTIVITY OF ETHANOLIC LEAF EXTRACT OF MORUS ALBA L. IN ANIMAL MODELS
Kidney stone is a common painful condition of multifactorial etiopathogenesis, and calcium oxalate (CaOx) is the commonest component of stone. The effect of the ethanolic leaf extract of Morus alba L. against Calculi-Pro...