Stability Indicating HPTLC method for Forskolin and Glycyrrhetinic acid
Journal Title: International Journal of Ayurveda and Pharmaceutical Chemistry - Year 2017, Vol 0, Issue 0
Abstract
A simple, specific and rapid high performance thin-layer chromatography (HPTLC) method was developed for quantification of Forskolin (FSK) and Glycyrrhetinic acid (GA) and also for the determination of stress degradation product as per the ICH guidelines. The compounds were chromatographed on precoated silica gel G 60254 plates using chloroform: methanol (9.5:0.5 v/v), as the mobile phase. The linear regression analysis of data for the calibration plots showed good linear relationship with R 2 = 0.996 and 0.991 for Glycyrrhetinic acid and Forskolin with respect to peak height and peak area, respectively, in the concentration range of 4–20 μg per spot of Glycyrrhetinic acid, and 40-200 μg per spot Forskolin. Mean recovery for Forskolin and Glycyrrhetinic acid was found to be 100.46% w/w, 100.87% w/w. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of Forskolin and Glycyrrhetinic acid in pharmaceutical dosage forms as well as their degradation products hence, it can be employed for routine analysis and as a stability-indicating method
Authors and Affiliations
Mrinalini Damle, Sneha Lonkar
Keywords
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