STABILITY-INDICATING METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ROSUVASTATIN CALCIUM IN BULK AND TABLET FORMULATION BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 8
Abstract
Objective: The present work was focused on the development and validation of reverse-phase high-performance liquid chromatography (RP-HPLC) method which is simple, rapid, precise, accurate, sensitive, economical, and stability indicating for the quantitation of rosuvastatin calcium in bulk and tablet formulation. Methods: The separation was attained on Waters Symmetry C18 column with dimensions 150×4.6 mm, 5 mm particle size employing 0.1% orthophosphoric acid buffer:acetonitrile in the ratio of 55:45% v/v as mobile phase, which was pumped at a rate of 1.0 ml/min and detected at a wavelength of 241 nm. Results: The linearity of the method was demonstrated in the concentration range of 2–12 μg/ml for rosuvastatin calcium with a correlation coefficient (r2) of 0.999, percentage drug recovery was found to be 100.22–101.16%, and percentage relative standard deviation <2. Limit of detection and limit of quantitation values were found to be 0.013 μg/ml and 0.042 μg/ml, respectively, and assay of marketed tablet formulation was found to be 99.76%. Conclusion: The developed RP-HPLC method was found to be simple, specific, sensitive, rapid, linear, accurate, precise, and economical and could be used for regular quality control of rosuvastatin calcium in bulk and tablet formulation.
Authors and Affiliations
SAILAJA B , SRAVANA KUMARI K
Keywords
Related Articles
- EP ID EP636173
- DOI -
- Views 151
- Downloads 0
A STUDY OF MULTIORGAN DYSFUNCTION IN PATIENTS WITH DENGUE AND ITS CLINICO- HAEMATOLOGICAL CORRELATION WITH SEVERITY.
Objective: To study of multiorgan dysfunction in patients with dengue and to correlate the severity and clinical outcome with different haematological parameters.Material & methods: All suspected cases of dengue admi...
CAROTID ARTERY INTIMA-MEDIA THICKNESS AND 10-YEAR RISK OF HEART DISEASE IN DIABETIC PATIENTS: A COMPARATIVE STUDY
Objective: The aim of the present study was to assess the factors affecting carotid artery intima-media thickness (CIMT) and 10-year risk of heart disease in diabetic patients classified according to CIMT. Methods: This...
ANALYSIS OF THE ANTIOXIDANT PROPERTY, CYTOTOXICITY AND ANTI-TUMOUR EFFICIENCY OF BAUHINIA PHOENICEA.
Objective: Bauhinia phoenicea Wight and Arn. is a medicinal plant endemic to Southern Western Ghats. In the traditional systems of medicine, it is using against various ailments including some oxidative disorders. Detail...
EVALUATION OF ANXIOLYTIC EFFECT OF MELILOTUS OFFICINALIS EXTRACTS IN MICE
Objective: Anxiety is one of the most common and serious mental illness affecting humankind and its extensiveness is on the rise at an alarming rate. Anxiolytic substances are highly acclaimed in the ranking of the most...
EVALUATION OF THE HISTOPATHOLOGICAL AND BIOCHEMICAL EFFECT OF ALOE VERA AQUEOUS EXTRACT ON DIABETES MELLITUS INDUCED BY STREPTOZOTOCIN IN RATS
Objective: Numerous plants have exciting pharmaceutical activities, but Aloe vera (AV) is likely to have many medicinal applications worldwide. Therefore, the present study focused on identifying the potent...