STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTIFICATION OF ISOPROTERENOL HCL IN BULK AND ITS FORMULATION BY RP-HPLC USING PDA DETECTION
Journal Title: Journal of Pharmaceutical and Scientific Innovation - Year 2018, Vol 7, Issue 5
Abstract
Theobjective of the present study was to develop an accurate, precise stability indicating reverse phase liquid chromatographic method to quantify Isoproterenol HCl in bulk and its formulation using PDA detection. The chromatographic separation of the analyte from the degradants was achieved on Phenomenex Luna columnwith mobile phase composition of Methanol and 0.1% Triethyl amine [pH 7.0], [20:80% v/v]at flow rate of 1.0 ml/min. The analyteseparation was monitored using PDA detection at 279nm. The method was linear in the concentration range of 10-60μg/ml.The method shown acceptablepercent relative deviations for the Inter-day (0.58%) and Intra-day (0.65%) precisions. The mean percent recovery for accuracy study was within the limit. From the stability studies method has proven specificity to quantify Isoproterenol HCl in presence of its degradation products.The method shown acceptance as according to ICH guidelines.
Authors and Affiliations
S. Muneer, Hindustan Abdul Ahad, Kothapalli Bonnoth Chandrasekhar
Keywords
Related Articles
- EP ID EP631503
- DOI 10.7897/2277-4572.075105
- Views 72
- Downloads 0
A REVIEW ON STANDARD MILK HYGIENE PRACTICES IN INDIA WITH SPECIAL REFERENCE TO CLEAN MILK PRODUCTION
Milk is the complete food. It contains in a balanced form of all the necessary and digestible elements for building and maintaining the human and animal body. In addition, it contains immunoglobulins which protect the ne...
A HURDLE APPROACH FOR PRESERVATION OF SEA FOOD PRODUCTS USING DIFFERENT STORAGE FACTORS WITH SYNERGISTIC BACTERIOCIN
Bacteriocin varies from most traditional medicines in being proteinaceous factors that are quickly assimilated by proteases in the human digestive system. They are ribosomal integrated peptides, and this condition develo...
STUDY OF THE ANTI-INFLAMMATORY ACTIVITY OF CRUDE DRUG FORMULATION WITH COTTON PELLETS INDUCED GRANULOMA IN RATS (CHRONIC INFLAMMATION)
The effect of crude drug anti-haemorrhoidal formulation on chronic inflammation in rats was studied. Cotton pellet-induced granuloma in rats is a sub-chronic model of inflammation and is sensitive to the treatment with t...
MOLECULAR DOCKING STUDY OF SOME NOVEL IMIDAZO[2,1-b] [1,3,4]-THIADIAZOLE DERIVATIVES
The molecular docking studies were performed on imidazo[2,1-b] [1,3,4] – thiadiazole derivatives using Pantothenatesynthetase, enoyl-acyl carrier protein reductase (InhA) and Aminoglycoside 2'-N-acetyltransferase protein...
OPTIMIZATION AND VALIDATION OF THE SIMULTANEOUS DETERMINATION OF VILDAGLIPTIN AND METFORMIN HCL IN HUMAN PLASMA BY RP-HPLC METHOD
A new and simple method was developed for quantitative determination of Vildagliptin (VILD) and Metformin (MET) spiked with plasma using an Onyx C18 Monolithic column connected with an Onyx C18 guard cartridgeand PDA det...