STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTIFICATION OF ISOPROTERENOL HCL IN BULK AND ITS FORMULATION BY RP-HPLC USING PDA DETECTION
Journal Title: Journal of Pharmaceutical and Scientific Innovation - Year 2018, Vol 7, Issue 5
Abstract
Theobjective of the present study was to develop an accurate, precise stability indicating reverse phase liquid chromatographic method to quantify Isoproterenol HCl in bulk and its formulation using PDA detection. The chromatographic separation of the analyte from the degradants was achieved on Phenomenex Luna columnwith mobile phase composition of Methanol and 0.1% Triethyl amine [pH 7.0], [20:80% v/v]at flow rate of 1.0 ml/min. The analyteseparation was monitored using PDA detection at 279nm. The method was linear in the concentration range of 10-60μg/ml.The method shown acceptablepercent relative deviations for the Inter-day (0.58%) and Intra-day (0.65%) precisions. The mean percent recovery for accuracy study was within the limit. From the stability studies method has proven specificity to quantify Isoproterenol HCl in presence of its degradation products.The method shown acceptance as according to ICH guidelines.
Authors and Affiliations
S. Muneer, Hindustan Abdul Ahad, Kothapalli Bonnoth Chandrasekhar
Keywords
Related Articles
- EP ID EP631503
- DOI 10.7897/2277-4572.075105
- Views 40
- Downloads 0
PHOSPHATE TYPE KIDNEY STONE (BRUSHITE) FORMATION IN GEL: A MORPHOLOGICAL STUDY ON GROWTH PATTERNS
Brushites are most commonly found phosphate stones in the kidneys, ureters and urinary bladder. The study was conducted to observe the possible growth patterns of brushite crystals in gel for 14 days. The crystals format...
ANIN VITROSTUDYOF AMELIORATIVE EFFECT OF COMBINATION OF ROOT EXTRACT OFOROXYLUM INDICUMWITH COW URINE DISTILLATE ON CYPERMETHRIN INDUCED CYTOTOXICITY ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES USING MTT ASSAY
Cypermethrin is a pesticide which is used by the farmers of Jabalpur as well as throughout the country. Farmers are exposed to it while spreading it on the crops. In the current study, effect of combination of root extra...
ROLE OF NANOBIOTECHNOLOGY IN PHARMACY AND MEDICINE: A REVIEW
Nanobiotechnology is a term, which describes the combination of the two different worlds of engineering and molecular biology. It is a fusion of the following words: “nano” meaning very small, “bio” meaning living, and “...
CURRENT REVIEW ON DENGUE : LIFE CYCLE OF DENGUE, PATHOPHYSIOLOGY AND DIAGNOSIS
Dengue is the major health concern in developing countries. Dengue is mainly caused by four serotypes of dengue virus (DENV). Based on the symptoms dengue is classified into febrile fever, dengue hemorrhagic fever (DHF)...
DRUG UTILIZATION EVALUATION AND ASSESSMENT OF ECONOMIC BURDEN OF ANTICOAGULANTS IN A TERTIARY CARE HOSPITAL IN SOUTH INDIA
Anticoagulants used for many indications, both prophylaxis and therapeutic treatment, are associated with high risk, hence drug utilization evaluation is essential. Objective: The objectives of the study were to evaluate...