Stability indicating RP HPLC method for determination of levitiracetam in pharmaceutical formulation
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2013, Vol 1, Issue 4
Abstract
The article reports on a development of RP-HPLC method for the quantitative determination of Levetiracetam in tablet dosage forms. The chromatographic separations were performed using Phenomenex C18 (250 mm x 4.6 mm i.d, 5 μm particle size) column at 40 ºC temperatures. The optimum mobile phase consisted of methanol, water and acetonitrile in the ratio of 30:10:60. Auto sampler 20 μl was used and kept at 15 ºC temperature. Analysis was done with flow rate of 1.0 ml/min at 212 nm ( max of Levetiracetam) wavelength by using photodiode array (PDA) detector. The drug was analyzed for acid, alkaline, oxidative, hydrolytic, photolytic and thermal degradation studies. The standard calibration curve was plotted for the drug and results showed that the drug was linear (r2 = 0.999) in the concentration range between 0.01 – 1.5 μg/ml. The results of stress testing undertaken according to the International Conference on Harmonization (ICH) guidelines reveal that the selected method is selective and stability-indicating for determination of levitiracetam in pharmaceutical formualtion.
Authors and Affiliations
M. Krishna Chaitanya Prasad| St. Ann’s college of Pharmacy, Andhrapradesh, Chirala, India, G. Vidhya Sagar| Veerayatan institute of Pharmacy, Bhuj, Kutth, Gujarat, India, P. Sudhakar| Department of biotechnology, ANU, Nagarjuna nagar, Guntur, India
Keywords
Related Articles
- EP ID EP13762
- DOI -
- Views 343
- Downloads 18
Formulation and Evaluation of sustained release matrix tablets of Glipizide
The aim of present investigation was to enhance the solubility of glipizide (BCS Class II). Glipizide is an oral antidiabetic agent with relatively short elimination half life. Inclusion complex of Glipizide with -cyc...
Nanoemulsion: A Review on Novel Profusion in Advanced Drug Delivery
Nanoemulsions are submicron sized emulsion that is under extensive investigation as drug carriers for improving the delivery of therapeutic agents. These are by far the most advanced nanoparticle systems for the system...
Bacteriostatic activity of con a lectin from Canavalia ensiformis
The aim of this work was to explore the therapeutic applications of Con A lectin from Canavalia ensiformis and to explore its antibacterial activity. Activity of lectin was quantified by their ability to agglutinate er...
Antioxidant and antimicrobial activity of whole seed extracts of Perseaamericana Mill
Perseaamericana Mill used in traditional medicine for the treatment of various ailments and has antibacterial, antifungal, anti-viral and wound-healing properties. In the present study, antioxidant and antibacterial ac...
Preliminary Pharmacological Screening of Ethanolic Extract of Aristoochia indica on Nephrolithiasis Rat
The effect of ethanolic extract of Aristolochia indica was studied on experimentally induced nepharolithatic and urolithuasis in rats. Oxalate urolithiasis was produced by the addition of 0.75% ethylene glycol in the d...