Stability indicating RP HPLC method for determination of levitiracetam in pharmaceutical formulation
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2013, Vol 1, Issue 4
Abstract
The article reports on a development of RP-HPLC method for the quantitative determination of Levetiracetam in tablet dosage forms. The chromatographic separations were performed using Phenomenex C18 (250 mm x 4.6 mm i.d, 5 μm particle size) column at 40 ºC temperatures. The optimum mobile phase consisted of methanol, water and acetonitrile in the ratio of 30:10:60. Auto sampler 20 μl was used and kept at 15 ºC temperature. Analysis was done with flow rate of 1.0 ml/min at 212 nm ( max of Levetiracetam) wavelength by using photodiode array (PDA) detector. The drug was analyzed for acid, alkaline, oxidative, hydrolytic, photolytic and thermal degradation studies. The standard calibration curve was plotted for the drug and results showed that the drug was linear (r2 = 0.999) in the concentration range between 0.01 – 1.5 μg/ml. The results of stress testing undertaken according to the International Conference on Harmonization (ICH) guidelines reveal that the selected method is selective and stability-indicating for determination of levitiracetam in pharmaceutical formualtion.
Authors and Affiliations
M. Krishna Chaitanya Prasad| St. Ann’s college of Pharmacy, Andhrapradesh, Chirala, India, G. Vidhya Sagar| Veerayatan institute of Pharmacy, Bhuj, Kutth, Gujarat, India, P. Sudhakar| Department of biotechnology, ANU, Nagarjuna nagar, Guntur, India
Keywords
Related Articles
- EP ID EP13762
- DOI -
- Views 338
- Downloads 18
Direct multiple shoots proliferation of black night shade (Solanum nigrum l.) from shoot tip explants induced by thidiazuron
The present investigation was undertaken in Black Night Shade (Solanum nigrum L.) which is an important medicinal plant. Direct multiple shoots proliferation was achieved from shoot tip. The shoot tips were cultured on...
A review on nanoparticles – preparation and evaluation parameters
Nanotechnology defined as a tiny science. Design characterization, production and applications of structures, devices and systems by controlling shape and size at nanometer scale is refers to nanotechnology. Nanotechnolo...
Flavonoid Contents From Some Exotic Tree Species Growing In Rajasthan Desert
Evaluation of flavonoid contents from four selected exotic tree species like Colophospermum mopane, Holoptelea integrifolia, Kigelia pinnata and Putranjiva roxburghii growing in Rajasthan Desert was carried out. The lea...
Atypical presentations of congenital cutaneous candidiasis- presenting a cohort of 8 cases
Congenital cutaneous candidiasis is an extremely rare disorder that usually presents within the first 6 days of life. We report 8 neonates who presented with generalized skin eruptions within first 6 days of life, char...
Tissue esterases of Lamellidens corrianus (Fresh water mussel)
Polyacrylamide gel electrophoretic (PAGE) technique was used to examine the esterase pattern of Ctenidia, Hepatopancreas, Hemolymph, Intestine, Mantle. Foot of the Lamellidens corrianus. The zymograms revealed the var...