Stability indicating RP HPLC method for determination of levitiracetam in pharmaceutical formulation
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2013, Vol 1, Issue 4
Abstract
The article reports on a development of RP-HPLC method for the quantitative determination of Levetiracetam in tablet dosage forms. The chromatographic separations were performed using Phenomenex C18 (250 mm x 4.6 mm i.d, 5 μm particle size) column at 40 ºC temperatures. The optimum mobile phase consisted of methanol, water and acetonitrile in the ratio of 30:10:60. Auto sampler 20 μl was used and kept at 15 ºC temperature. Analysis was done with flow rate of 1.0 ml/min at 212 nm ( max of Levetiracetam) wavelength by using photodiode array (PDA) detector. The drug was analyzed for acid, alkaline, oxidative, hydrolytic, photolytic and thermal degradation studies. The standard calibration curve was plotted for the drug and results showed that the drug was linear (r2 = 0.999) in the concentration range between 0.01 – 1.5 μg/ml. The results of stress testing undertaken according to the International Conference on Harmonization (ICH) guidelines reveal that the selected method is selective and stability-indicating for determination of levitiracetam in pharmaceutical formualtion.
Authors and Affiliations
M. Krishna Chaitanya Prasad| St. Ann’s college of Pharmacy, Andhrapradesh, Chirala, India, G. Vidhya Sagar| Veerayatan institute of Pharmacy, Bhuj, Kutth, Gujarat, India, P. Sudhakar| Department of biotechnology, ANU, Nagarjuna nagar, Guntur, India
Keywords
Related Articles
- EP ID EP13762
- DOI -
- Views 315
- Downloads 18
Evaluaton of hypoglycaemic activity of Aegel marmelos alcoholic seed extract in experimental models of hyperglycemic rats
The present study aimed to investigate the antidiabetic effect of alcoholic seed extract of Aegel marmelos in comparison with glibenclamide in in vivo using alloxan rat model.The diabetic rats were orally given glibenc...
Vegetation dynamics between an open patch and a forest edge
The study was conducted in two adjacent open area (patches and forest edges) resulted from a major blowdown as a laboratory exercises in SFI 223 (Advanced Silviculture). The two areas were utilized to understand the gr...
Expulsion by Ionic Complexation: Benchmark Therapy for Atherosclerosis A Review
Ethylenediamine tetraacetic acid (EDTA) chelation therapy has been practiced since longtime for the treatment of cardiovascular diseases, alone or in combination with other treatments. It has been recommended as a harm...
Studies on antidiabetic activity of Acacia ferruginea DC. stem bark
The methanol extract of Acacia ferruginea (Family- Mimosaceae) was studied for possible antidiabetic activity on normoglycaemic, OGTT and alloxan induced diabetic rats at doses of 100, 200, and 400 mg/kg, p.o. The acut...
Cyclic Changes In The Hypothalamo Neurohypophysial System of Xenentodon Cancila (HAM.)
The presence of functional hypothalamo-hypophysial neurosecretory system in vertebrates has led to revise earlier views regarding the hypothalamus-pituitary relationship. The hypothalamo-neurohypophysial system which c...