Stability Indicating RP-HPLC Method for Simultaneous Estimation of Atazanavir and Cobicistat in Tablets
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 11, Issue 6
Abstract
Aim: The aim of this study was to develop a stability indicating RP-HPLC method for simultaneous quantitative analysis of atazanavir and cobicistat in tablets. Place and Duration of Study: At Rainbow Pharma Training Lab, Kukatapally, Hyderabad, India and Department of chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, India in between October 2015 and February 2016. Methodology: Atazanavir and cobicistat was eluted on the Inertsil C8, 150 mm x 4.6 mm, 5 µm analytical column with a mobile phase consisting of 0.1 M ammonium acetate and methanol (50:50 v/v), pumped at 1.2 mL/min flow rate. The column was maintained at 30°C and 10 μl of the solutions were injected. UV detection was performed at 234 nm. According to ICH guidelines, the method was validated. Results: Under the optimized chromatographic conditions the retention times of atazanavir and cobicistat were 2.559 min and 3.576 min, respectively. Linearity was observed in the concentration range of 45-135 μg/mL for atazanavir and 22.5-67.5 μg/mL for cobicistat. The percent recovery and percent relative standard deviation for both the drugs were in the range of 99.311-100.342% and 0.290-0.401%, respectively. The results of forced degradation studies demonstrated the stability-indicating power of the method. Conclusion: The proposed method was found to be appropriate for the quality control of atazanavir and cobicistat hydrochloride simultaneously in a bulk drug as well as in a pharmaceutical dosage forms.
Authors and Affiliations
M. Venkata Siva Sri Nalini, B. Haribabu, P. Rama Krishna Veni
Ameliorative Effects of Alcohol on Human Diabetic Volunteers – A Prospective Study
Aims: The purpose of this study is to assess and confirm the ameliorative effects of alcohol consumption on biochemical indices of blood i.e., blood glucose, HbA1c, NO2, NO3, lipid profiles, hs-CRP (high sensitive C–Reac...
Synergistic Activity of the Antibiotic Meropenem in Combination with Edible Mushroom Extracts against Multidrug Resistant Bacteria
Aims: The objective of this study was to evaluate the antibacterial potency of meropenem in synergism with five edible mushroom extracts against multidrug resistant clinical isolates. Methodology: Minimum Inhibitory Con...
Effect of Solvent and Crystallization Method on Physicochemical Properties of Aceclofenac and Fenofibrate
Aims: Aim of this study was to prepare Aceclofenac and Fenofibrate crystals by cooling crystallization and non-cooling crystallization methods using various solvents. Also to study the effect of crystallization condition...
Anti-proliferative Effect of Chickpea Extract on Hela, MCF-7, Saos and Fibroblast Cell Lines
Background and Aim: The present study aimed to investigate the proliferation inhibitory effects of seed protein extract from chickpeas (Cicer arietinum L.) on cancer cell lines. Materials and Methods: The chickpea water...
A Comparative Analysis of Electronic Prescribing Near Misses in King Saud Medical City, Riyadh, Saudi Arabia
Background: A “near miss” or close call is a medication error that happened but did not result in injury or damage to the patient. These medication errors (MEs) are captured and corrected before affecting the patient eit...