STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ATOVAQUONE AND PROGUANIL IN BULK AND TABLET DOSAGE FORM
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 12
Abstract
The present work aimed to develop a stability indicating high Performance Liquid Chromatographic (HPLC) validated method for the cestimation of combined tablet formulation of Atovaquone and Proguanil Hydrochloride. Chromatographic separation was optimized by gradient HPLC on a C18 column [Inertsil Silica, 250 x 4.6 mm, 5μ] utilizing a mobile phase consisting a mixture of 10mM ammonium formate, pH 3.5 and 90:10 v/v acetonitrile – methanol in the ratio of 30:70 v/v at a flow rate of 0.9ml/min with UV detection at 254nm. The retention time of Atovaquone and Proguanil Hydrochloride was 7.3 min and 3.8 min respectively. The developed method was validated as per ICH guidelines. Linearity of the method was good over the concentration range 2.5μg/ml to 20μg/ml for proguanil and 6.25-50 μg/ml for atovaquone. Correlation coefficient was found to be 0.999&0.999 for proguanil & Atovaquone respectively. The % mean recovery of proguanil (98.38-101.09.%) & atovaquone (98.62-100.99%) at each level was within the limits of 98% and 102%.The results obtained for accuracy, precision, LOD, LOQ and Ruggedness were within the limits. Thus the validated economical method was applied for forced degradation study of Atovaquone and Proguanil Hydrochloride tablet.
Authors and Affiliations
S. Naazneen
Keywords
Related Articles
- EP ID EP618304
- DOI -
- Views 128
- Downloads 0
PREVALENCE AND RISK FACTORS OF NON-ADHERENCE IN PSYCHIATRIC PATIENTS IN TAIF, SAUDI ARABIA
Objectives: To estimate the prevalence of non-adherence to medication among and to explore the different reasons for drug non adherence among patient in mental health hospital in Taif city. Subjects and methods: It is a...
ASSESSMENT OF IRON PROFILE IN SUDANESE KIDNEY POSTTRANSPLANT PATIENTS -KHARTOUM STATE
Background: Posttransplantation anemia (PTA) is a common obstacle for kidney post-transplant patients and a risk factor for many complicated consequences. Exposure to the immunosuppressive drug has a role on iron profile...
ANALYTICAL STUDY ON ASTHIVAHA SROTAS & ITS APPLIED ASPECT
“Dosha Dhatu Mala Mulam Hi shariram” which means that Vataadi Tridosha, Rasadi Sapta Dhatu & Trimala (Mutra, Purish, Sweda) are the building blocks of human body. For a healthy body each individual should have Sama Prama...
PROTECTIVE EFFECT OF ALLOPRINOL, NIFEDIPIN, VITAMIN A AGAINST CCL4 INDUCED ACUTE LIVER INJURY IN EXPERIMENTAL RABBIT MODEL
Objective: The present study was designed to determine the hepatoprotective effect of well Known drugs Allopurinol, Nifedipine and Vitamin-A in acute liver injury induced by Toxic agent CCL4. Materials & Methods: The nor...
PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF THE METHANOL LEAF EXTRACT OF LUFFA CYLINDRICA ON CERTAIN PATHOGENIC MICROBES.
The attention of the pharmaceutical scientists has been drawn to studies on the potential antimicrobial activities of plant derived substances, an untapped source of antimicrobials which are used in traditional medicine...