STANDARDISATION AND COMPARISON OF DIFFERENT METHODS FOR PREPARING PLATELET RICH PLASMA
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 90
Abstract
BACKGROUND Platelet rich plasma (PRP) is defined as a volume of the plasma fraction of autologous blood with an above baseline platelet concentration. Platelet releases the growth factors from their granules that are beneficial for tissue healing. Therefore, platelet rich plasma is being increasingly used in various medical and surgical fields. As such, there are no defined guidelines available for PRP preparation. Hence, this study is aimed to develop a protocol for an optimised method of PRP preparation by standardisation of two different methods, namely tube method and syringe method in our setup. MATERIALS AND METHODS The prospective comparative study was conducted at MGM Blood Bank, Kamothe, Navi Mumbai, for a period of 3 months. Blood samples were collected from 10 voluntary healthy donors. PRP was prepared by double centrifugation method. Four protocols of PRP preparation were defined for both tube and syringe method each. Platelet count and platelet increment were analysed and tabulated. RESULTS The reliable, practical and cost-effective protocol for platelet rich plasma preparation in our setup was found to be 1200 rpm for 12 mins in first spin and 2000 rpm for 10 mins in second spin for tube method and 900 rpm for 8 mins in first spin and 2500 rpm for 10 mins in second spin for syringe method in our setup. CONCLUSION With some limitations, our study determined the protocols for PRP preparation by two simple and cost-effective techniques. Syringe method was found more effective in terms of platelet increment and sterility in our setup. Therefore, the study may contribute towards future researches where a defined protocol of PRP preparation and clinical benefits are still needed to be explored.
Authors and Affiliations
Lakshita Varshney, Seema Gupta
Keywords
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- EP ID EP278257
- DOI 10.14260/jemds/2017/1376
- Views 73
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