STANDARDISATION AND COMPARISON OF DIFFERENT METHODS FOR PREPARING PLATELET RICH PLASMA
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 90
Abstract
BACKGROUND Platelet rich plasma (PRP) is defined as a volume of the plasma fraction of autologous blood with an above baseline platelet concentration. Platelet releases the growth factors from their granules that are beneficial for tissue healing. Therefore, platelet rich plasma is being increasingly used in various medical and surgical fields. As such, there are no defined guidelines available for PRP preparation. Hence, this study is aimed to develop a protocol for an optimised method of PRP preparation by standardisation of two different methods, namely tube method and syringe method in our setup. MATERIALS AND METHODS The prospective comparative study was conducted at MGM Blood Bank, Kamothe, Navi Mumbai, for a period of 3 months. Blood samples were collected from 10 voluntary healthy donors. PRP was prepared by double centrifugation method. Four protocols of PRP preparation were defined for both tube and syringe method each. Platelet count and platelet increment were analysed and tabulated. RESULTS The reliable, practical and cost-effective protocol for platelet rich plasma preparation in our setup was found to be 1200 rpm for 12 mins in first spin and 2000 rpm for 10 mins in second spin for tube method and 900 rpm for 8 mins in first spin and 2500 rpm for 10 mins in second spin for syringe method in our setup. CONCLUSION With some limitations, our study determined the protocols for PRP preparation by two simple and cost-effective techniques. Syringe method was found more effective in terms of platelet increment and sterility in our setup. Therefore, the study may contribute towards future researches where a defined protocol of PRP preparation and clinical benefits are still needed to be explored.
Authors and Affiliations
Lakshita Varshney, Seema Gupta
Keywords
Related Articles
- EP ID EP278257
- DOI 10.14260/jemds/2017/1376
- Views 71
- Downloads 0
A HISTOMORPHOLOGICAL STUDY OF PAEDIATRIC ADRENAL TUMOURS
BACKGROUND: Tumors of the adrenal gland are extremely rare in children. Those of the adrenal cortex account for only 0.3-0.4 % of all childhood neoplasms. Its rarity has hindered the identification of meaningfu...
CLINICAL AND HAEMATOLOGICAL EVALUATION OF LEUKAEMIAS, USING CYTOCHEMICAL STAINS AND IMMUNOPHENOTYPING
BACKGROUND Leukaemias are characterised by abnormal proliferation of haematopoietic cells, causing progressive infiltration of the marrow. It is the eleventh most common cancer in the world, and increasingly found now. T...
EVALUATION OF THE EFFECTS OF CAPSULES CONTAINING TRIBULUS TERRESTRIS EXTRACT AND LCARNITINE ON TREATMENT OF OLIGOSPERMIA IN MALES
BACKGROUND Infertility is defined as failure to achieve pregnancy after one year of unprotected sexual intercourse. Male infertility is one of the most challenging problems in andrology. The common cause of male infertil...
COMBINED TIBIAL AVULSION OF ANTERIOR AND POSTERIOR CRUCIATE LIGAMENT OF KNEE JOINT: A CASE REPORT
Avulsion fractures of tibial intercondylar eminence is a rare injury mainly affecting the pediatric population between 8 to 14 and is even rarer in adults with very few cases reported in literature. It occurs...
THERAPEUTIC EFFECTS OF CARBOGEN THERAPY IN SUDDEN SENSORINEURAL HEARING LOSS
BACKGROUND Sudden Hearing Loss (SHL) is a rare disease accounting for 1% of all sensorineural hearing loss cases, but it is considered an otological emergency. The sooner the treatment begins, the outcome proves to be be...