STANDARDIZATION OF MARKETED AMRITARISHTA- A HERBAL FORMULATION
Journal Title: International Journal of Pharmacognosy - Year 2016, Vol 3, Issue 9
Abstract
The present study aims to standardize Amritarishta based upon chromatographic and spectral studies. The spectral data and HPTLC fingerprint of ethanolic extract of Amritarishta could be used as a valuable analytical tool in the routine standardization of Amritarishta to check the batch to bath variation. Fraction I and II of ethanolic extract of Amritarishta and standard Luteolin and Apigenin were compared by TLC, HPTLC and HPLC analysis to evaluate the presence of Luteolin and Apigenin employing Toluene: Ethyl acetate: Glacial acetic acid (5:4:1 v/v/v) , as a mobile phase respectively. The Rf values (0.64) and (0.81) for Luteolin and Apigenin in both sample and reference standard were found comparable under UV light at 366 nm respectively. The high performance thin layer chromatography method developed for quantization was simple, accurate and specific. The present standardization provides specific and accurate tool to develop qualifications for identity, transparency and reproducibility of biomarkers Luteolin and Apigenin in liquid formulation.
Authors and Affiliations
Kiran A. Wadkar et al.
Keywords
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