STANDARDIZATION OF MARKETED AMRITARISHTA- A HERBAL FORMULATION
Journal Title: International Journal of Pharmacognosy - Year 2016, Vol 3, Issue 9
Abstract
The present study aims to standardize Amritarishta based upon chromatographic and spectral studies. The spectral data and HPTLC fingerprint of ethanolic extract of Amritarishta could be used as a valuable analytical tool in the routine standardization of Amritarishta to check the batch to bath variation. Fraction I and II of ethanolic extract of Amritarishta and standard Luteolin and Apigenin were compared by TLC, HPTLC and HPLC analysis to evaluate the presence of Luteolin and Apigenin employing Toluene: Ethyl acetate: Glacial acetic acid (5:4:1 v/v/v) , as a mobile phase respectively. The Rf values (0.64) and (0.81) for Luteolin and Apigenin in both sample and reference standard were found comparable under UV light at 366 nm respectively. The high performance thin layer chromatography method developed for quantization was simple, accurate and specific. The present standardization provides specific and accurate tool to develop qualifications for identity, transparency and reproducibility of biomarkers Luteolin and Apigenin in liquid formulation.
Authors and Affiliations
Kiran A. Wadkar et al.
Keywords
Related Articles
- EP ID EP428484
- DOI -
- Views 91
- Downloads 0
APPLICATION OF MOLECULAR BEACON BASED BIOSENSOR AGAINST rs699 SNP IN HYPERTENSIVE AND NON- HYPERTENSIVE PUNJABI POPULATION
Molecular beacons have shown their topnotch potential in a variety of basic research, biomedical detection and in clinical diagnosis. Their excellent selectivity, sensitivity, detection without separation have made them...
CADMIUM CHLORIDE INDUCED ELECTROLYTE IMBALANCE, PANCYTOPENIA, OXIDATIVE STRESS AND RENAL DAMAGE: AMELIORATING EFFECTS OF AQUEOUS EXTRACT OF TELFAIRIA OCCIDENTALIS
Cadmium is a known nephrotoxic metal with other toxicities including pancytopenia. Telfairia occidentalis is a medicinal plant with antioxidant and heamoprotective properties. This study was carried out to investigate th...
POLY HERBAL THERAPY IN OINTMENT BASE FOR WOUND HEALING ACTIVITY IN RATS
In the present study, an indigenous herbal formulation containing the ethanolic extracts of rhizomes of Curcuma longa, fruits of Terminalia chebula, leaves of Azadirachta indica, leaves of Lawsonia inermis and leaves of...
MAHUA OIL CONTAINING SUPPOSITORY BASE EXHIBITED HIGHER DRUG RELEASE AS COMPARED TO COCOA BUTTER BASE
The present research aimed at scrutinizing the role of Mahua Oil (MO) as a suppository base which can enhance the release of drug in the site of administration. Three formulations were designed with erythromycin as the d...
EFFECT OF DESMOSTACHYA BIPINNATA EXTRACTS ON CASTOR OIL AND MAGNESIUM SULPHATE INDUCED DIARRHEA IN WISTAR RATS
Desmostachya bipinnata (L.) Stapf [Gramineae (Poaceae)] is used in Indian traditional medicines system for treatment of various diseases such as asthma, kidney stone, diarrhea, wound healing, etc. These traditional claim...