Standardization of process for increased production of pure and potent tetanus toxin
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2013, Vol 3, Issue 3
Abstract
When stationary pot culture was replaced by submerged cultivation of Clostridium tetani, an anaerobic organism, in a fermentor using a vibromixer and optimum supply of sterile air to the headspace of the fermentor to flush out the accumulated gases, a significant increase in the tetanus toxin yield in a short time cultivation (about 5 to 6 days against 8 days) was noticed. It was found that under optimal conditions of temperature, vibromixing, surface aeration, and an alkaline pH favored toxin release. Furthermore, to enhance the production volume, fermentor culture is more suitable. The tetanus toxin was produced with good Limes flocculation (Lf) titre and high antigenic purity. Under optimal conditions, the papain digest broth was successfully substituted in place of N.Z Case for the production of pure and potent tetanus toxin. J Microbiol Infect Dis 2013; 3(3): 133-139 Key words: Clostridium tetani, modified mueller miller medium, papain digest, limes flocculation
Authors and Affiliations
Chellamani Muniandi, Premkumar Lakshmanan, Kavaratty Mani, Subashkumar Rathinasamy
Keywords
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- EP ID EP120515
- DOI 10.5799/ahinjs.02.2013.03.0096
- Views 136
- Downloads 0
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