Starch Phosphorylase: An Overview of Biochemical Characterization, Immobilization and Peptide Mapping
Journal Title: Biotechnology Journal International - Year 2015, Vol 5, Issue 3
Abstract
Starch phosphorylase (EC 2.4.1.1) is the key enzyme which catalyzes the reversible conversion of starch and inorganic phosphate into glucose-1-phosphate (G-1-P) and has been reported in many higher plants. It can be exploited for industrial purposes for tailoring starch and for the synthesis of biopolymers which may be useful in food industry and are also of clinical importance. Starch in the form of insoluble granules accumulates in chloroplasts as the primary product of photosynthesis. Multiple forms of the enzyme have been reported in different plant tissues which have been predicted to have different roles in starch metabolism. Here, various biochemical properties have been reviewed. Various techniques for enzyme immobilization have been discussed. Besides, reports on immobilization of starch phosphorylase from different sources and on different solid matrices have also been reviewed. Peptide mapping reports of various proteins have also been assessed in this review.
Authors and Affiliations
Anil Kumar, Ritu Jain, Sarika Garg
Keywords
Related Articles
- EP ID EP237080
- DOI 10.9734/BBJ/2015/14522
- Views 157
- Downloads 0
RNA-Seq Evaluating Several Custom Microarrays Background Correction and Gene Expression Data Normalization Systems
Microarray gene expression technologies represents a widely used tool in transcriptomics and genomics studies worldwide. Even if this technology exhibits a low dynamic range as well as a feeble sensitivity and specificit...
Analysis of Gene Expression Associated with Copper Toxicity in White Birch (Betula papyrifera) Populations from a Mining Region
The Greater Sudbury Region (GSR) is one of the most ecologically disturbed regions of Canada. Recent studies have shown that Betula papyrifera accumulate metals in roots or leaves. The main objectives of the present stud...
Gradual Changes in Genetic Based Developments for Phylogenetic Trends in Picornaviruses
On the basis of the several techniques, the new groups and the strains within the Picornaviruses were classified. New groups like human pathogen EV22 (Echovirus 22) was discovered that was found to be highly different fr...
Evaluation of Mutagenic Effects of Monosodium Glutamate Using Allium cepa and Antimutagenic Action of Origanum majorana L. and Ruta chalepensis Medical Plants
At the present study, Allium cepa root tips were collected during 2,6 and 24 hrs after treated with selected concentrations (1.0,3.0,5.0 and 7.0 g/L) of Mono-Sodium Glutamate (MSG) that used as flavour enhancer in foods....
Optimizing Salting and Immersion Time in Liquid Smoke of Farmed Gilthead Sea Bream (Sparus aurata) Fillets Using Response Surface Methodology
Aims: To produce smoked gilthead sea bream fillets using the liquid smoke dipping method during brining and optimize the brining-smoking process using the response surface method. Study Design: The experimental design wa...