STATISTICAL, DIAGNOSTIC AND RESPONSE SURFACE ANALYSIS OF NEFOPAM HYDROCHLORIDE NANOSPHERES USING 35 BOX-BEHNKEN DESIGN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: Objective of the current investigation was to analyze effects of operating conditions on characteristics of nefopam hydrochloride nanospheres (NFH-NS). Statistical assessment and diagnostic analysis examined an adequacy and reliability of models.Methods: NFH-NS were developed by quasi solvent diffusion technique using poly (meth) acrylates by 35 Box-Behnken design. Drug: polymer ratio (X1), surfactant concentration (X2), stirring time (X3), DP/CP Ratio (X4) and stirring speed (X5) were selected as independent variables. Response variables investigated were % entrapment efficiency (% EE), mean particle size, % process yield and % drug loading (% DL).Results: Standardized Pareto chart illustrated that X1 and X5 were important factors (p<0.05) affecting response parameters of nanospheres. Significant model F-value (p<0.05) and non-significant lack of fit F-value (p>0.05) epitomized an accuracy of data. Smaller value of predicted residual error sum of squares (PRESS) for regression models stipulated good fit of models. Diagnostic analysis proved normality of data and signified that actual values of response parameters were in agreement with predicted values. Graphical analysis concluded that X1, X2, X4 and X5 had the significant positive effect on % EE. X1 and X5 produced remarkable synergistic and antagonistic effect on mean particle size, respectively. X1 and X5 exhibited positive effect on % process yield. X1 produced significant antagonistic effect on % DL.Conclusion: Optimization report concluded that formulation prepared with 1:3 drug: polymer ratio (w/w), 2 % (w/v) surfactant, 3.8 h stirring time, 1:12 DP/CP ratio and 2000 rpm stirring speed was having highest desirability function of 0.920. Regression models indicated good fit of model, adequate model discrimination and concluded that models can be used to navigate design space.Â
Authors and Affiliations
Sukhbir Singh, Yash Paul Singla, Sandeep Arora
Keywords
Related Articles
- EP ID EP578221
- DOI -
- Views 101
- Downloads 0
INVESTIGATION OF BIOGENIC SILVER NANOPARTICLES GREEN SYNTHESIZED FROM CARICA PAPAYA
Objective: This work mainly focuses on the investigation of optical, structural and morphological characteristics of Silver nanoparticles (Ag-NPs) synthesized using Papaya (Carica Papaya) leaf extract and to study the me...
ASSESSING THE THERAPEUTIC ROLE OF JOSHANDA: PHYTOCHEMICAL, ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITIES
Objective: Joshanda, a polyherbal Unani formulation is extensively used as a common home remedy for the treatment of a cough and cold accompanied by pharyngeal inflammation and fever. This study aimed to analyze phytoche...
EVALUATION OF ANTIBIOTIC AND BIOCHEMICAL POTENTIAL OF BRYOPHYTES FROM KUMAUN HILLS AND TARAI BELT OF HIMALAYAS
Objective: Today, one of the major problems in the treatment of disease is the development of resistance against conventional antibiotics. One way to curb the problem of increasing antibiotic resistance is to use botanic...
CURRENT STATUS ON BIOLOGICAL ACTIVITIES OF ACORUS CALAMUS - A REVIEW
The review emphasis on the current status of the researches in Acorus calamus highlighting their number of useful biological activities. The Acorus calamus most extensively investigated phytochemically and pharmacologica...
IN SILICO SCREENING OF HESPERETIN AND NARINGENIN ESTER DERIVATIVES AS ANTICANCER AGAINST P-GLYCOPROTEIN
Objective: Study the in silico P-Glycoprotein (P-GP) inhibition activity of hesperetin and naringenin ester derivatives. Acyl group substituent was different in the length of the carbon atom chain (acetyl, propionyl, but...