Statistical media optimization studies for growth and polydroxybutyrate (PHB) production by Pseudomonas spp.
Journal Title: Journal of Applied and Natural Science - Year 2016, Vol 8, Issue 3
Abstract
Using glucose as carbon source and mustard cake and yeast extract as nitrogen sources bacterial isolate Pseudomonas B2 exhibited a maximum PHB recovery of 0.620 (in terms of O.D.) and PHB weight of 0.27g/L in 96 h. To determine the possibility of growth potential of Pseudomonas spp., it was grown in different carbon sources like fructose, glucose, maltose, mannitol etc. and it was found that glucose yielded good growth and PHB production. In order to incorporate cost effective nitrogen and carbon source, mustard cake and cotton cake as nitrogen source and molasses as carbon were used in medium. Statistical media optimization design was used to optimize the culture conditions for maximizing the PHB production. A maximum of 0.37 g/L of PHB and 0.746 (O.D.) PHB recoveries were obtained using optimized concentrations. Batch kinetics can be used for model development, which will make possible simulation of nutrient limited cultivation(s) for over accumulation of PHB. FTIR studies confirmed the presence of PHB.
Authors and Affiliations
Mukesh R. Jangra, Ritu Batra, Ikbal Ikbal, Ankasha Jain, Rekha Ahlawat, Virendra K. Sikka
Keywords
Related Articles
- EP ID EP285731
- DOI -
- Views 94
- Downloads 0
Dawn singing behavior of a tropical bird species, the Pied Bush Chat Saxicola caprata
This paper aims to study the structure and pattern of dawn song in a tropical avian species, the Pied Bush Chat (Saxicola caprata) in Haridwar (290 55’ N, 780 08’ E; Uttarakhand, India) in 2009. Males delivered complex d...
Antibacterial activity and phytochemical screening of ethanolic leaf, stem and flower extract of Aerva lanata
Screening of phytochemicals provides the potentiality for serving several illnesses. The current investigation was carried out the antibacterial activity and phytochemical screening of ethanolic extract of leaf, flower a...
Response of different wheat varieties towards Azospirillum and phosphate solubilizing bacteria (PSB) seed inoculation
Present investigation was undertaken to utilize Azospirillum and Phosphate Solubilizing Bacteria (PSB) to avail atmospheric nitrogen and soluble phosphate in soil for the wheat crop. The sonalika variety (V1) responded b...
Effect of arbuscular mycorrhizal (AM) fungi inoculation on enzymatic activity and zinc uptake under direct seeded rice system
The application of treatment T3 (Glomus mosseae + 100 % RDF NK) produced significantly more root volume by 72.60 %, 17.80 %, 12.25 %, 14.13 % over the application of treatment T1 (Control), treatment T5 (Glomus coronatum...
Induction of genotoxicity after subchronic treatment with 4-nonylphenol in blood cells from gill and kidney and restoration of DNA integrity after recovery by Channa punctatus
The aim of present study was to assess the genotoxic effects of endocrine-disrupting compounds 4-nonylphenol (NP) on Channa punctatus after sub chronic exposure. Blood cells from gill and kidney were used for the study a...