Statistical media optimization studies for growth and polydroxybutyrate (PHB) production by Pseudomonas spp.
Journal Title: Journal of Applied and Natural Science - Year 2016, Vol 8, Issue 3
Abstract
Using glucose as carbon source and mustard cake and yeast extract as nitrogen sources bacterial isolate Pseudomonas B2 exhibited a maximum PHB recovery of 0.620 (in terms of O.D.) and PHB weight of 0.27g/L in 96 h. To determine the possibility of growth potential of Pseudomonas spp., it was grown in different carbon sources like fructose, glucose, maltose, mannitol etc. and it was found that glucose yielded good growth and PHB production. In order to incorporate cost effective nitrogen and carbon source, mustard cake and cotton cake as nitrogen source and molasses as carbon were used in medium. Statistical media optimization design was used to optimize the culture conditions for maximizing the PHB production. A maximum of 0.37 g/L of PHB and 0.746 (O.D.) PHB recoveries were obtained using optimized concentrations. Batch kinetics can be used for model development, which will make possible simulation of nutrient limited cultivation(s) for over accumulation of PHB. FTIR studies confirmed the presence of PHB.
Authors and Affiliations
Mukesh R. Jangra, Ritu Batra, Ikbal Ikbal, Ankasha Jain, Rekha Ahlawat, Virendra K. Sikka
Keywords
Related Articles
- EP ID EP285731
- DOI -
- Views 76
- Downloads 0
Induced breeding and larval rearing of Monopterus cuchia (Hamilton, 1822) under the agro-climatic conditions of Meghalaya, India
Monopterus cuchia is a fresh water eel widely distributed in Nepal, Myanmar, Pakistan but predominantly available in Bangladesh and North East Region of India. The population of the species has been declined in its natur...
Management of sheath blight in rice through application of Validamycin, Trichoderma harzianum and Pseudomonas fluorescence
Eleven different combinations of Trichoderma harzianum, Psedumonas fluorescens and Validamycin 3L, were applied at different crop stages viz. seed, seedling, tillering and symptoms initiation along with one application o...
Foraging behavior of major insect pollinators on pumpkin, Cucurbita moschata (Duch.ex Lam)
Foraging activity period of different honey bee species on C. moschata (C-1076) flowers at different day hours during August-September (2013) revealed that A. dorsata, A. mellifera, A. cerana and A. florea initiated thei...
Inheritance of powdery mildew resistance in sunflower (Helianthus annuss L.)
Powdery mildew caused by Golovinomyces cichoracearum is the most important disease on sunflower worldwide. The investigation was undertaken to determine the mode of genetic inheritance of powdery mildew resistance in fiv...
Impact of industrial effluents on ground water and soil quality in the vicinity of industrial area of Panipat city, India
The present paper is aimed towards the assessment of heavy metal contamination of agricultural soil due to irrigation with contaminated ground water affected by textile industrial effluents at Panipat city in India. Samp...