Stem Cell-like Molecular and Morphological Properties in a Subpopulation of Human Oral Cancer Cells
Journal Title: Journal of Oral Biology - Year 2016, Vol 3, Issue 1
Abstract
Background: Side population (SP) cells are present in many human cancers and cancer cell lines, including human oral cancer SCC-4 cells. SP cells were identified as a side branch of Hoechstlow cells existing in a fraction darker than cells in G0/G1 phase. The ratio of isolated SP cells accounted for approximately 1.5% of the SCC-4 cell population. Here, we describe the stem cell-like properties of this subpopulation of SCC-4 cancer cells. Methods: The expression levels of the stem cell markers OCT4, SOX2 and NANOG in SP cells were measured and compared with those of non-SP cells as indicators of pluripotency. Additionally, sphere formation ability and alkaline phosphatase activity were investigated as indicators of self-renewal and undifferentiated state, respectively. Results: OCT4 and NANOG levels were significantly higher in SP cells than in non-SP cells, while SOX2 levels did not differ between the two cell types. After induction for 2 weeks, sphere formation was notably observed and alkaline phosphatase activity was detected in spheres of SP cells. Conclusions: Our findings reveal that SP cells have stem cell-like properties including pluripotency, self-renewal, and retention of undifferentiated state. These results suggest that a subpopulation of oral cancer cells has the potential to act as stem cells. The stem cell-like properties revealed in this study could be useful in future applications targeting intractable cancer stem cells.
Authors and Affiliations
Tadashige Nozaki
Keywords
Related Articles
- EP ID EP205214
- DOI 10.13188/2377-987X.1000016
- Views 93
- Downloads 0
Periodontal Disease Linked to Erectile Dysfunction
Mouth acts as a window to a lot of systemic diseases and serves as a port of entry of the various infections that can alter and affect the immune status of the person [1]. Periodontitis is a destructive inflammatory dise...
Serpine1 Mediates Porphyromonas gingivalis Induced Insulin Secretion in the Pancreatic Beta Cell Line MIN6
Periodontitis is an inflammatory disease resulting in destruction of gingiva and alveolar bone caused by an exuberant host immunological response to periodontal pathogens. Results from a number of epidemiological studies...
Videoendoscopic Evaluation of Saliva Aspiration in a Municipal Hospital
Videoendoscopic examination of swallowing is useful to examine for aspiration of saliva and food at the bedside. The purpose of the present study was to compare saliva aspiration to aspiration of food in patients with dy...
A Guideline on Provisional Restorations for Patients Undergoing Implant Treatment
Implant therapy has been well documented to have high success rate to restore partially and fully edentulous patients [1,2]. With increased patient acceptance for implant treatment and demand for minimum disruption on th...
See Cavities, Think Systemic Disease
Caries are frequently a sign of excess sugar intake and this can be related to systemic disease. The clinician should broaden their thinking to include the possibility that excess sugar intake can cause systemic disease...