Stress Can Undermine the Process of Spermatogenesis in Mice
Journal Title: Jurnal Ners - Year 2011, Vol 6, Issue 2
Abstract
Introduction: This study aimed to determine the effect of stress on the process of spermatogenesis and testosterone levels of mice (Mus musculus) male. Method: This was an experimental study using a completely randomized design. The variables examined were the number of spermatogenic cells (spermatogonia, spermatocytes, and spermatids oval) and testoseron levels. Data analysis using oneway analysis of variance (ANOVA), test a small real difference (LSD) 5%, and the kruskal wallis test. This study used 36 mice (Mus musculus) male age 2 months with 20–40 gram body weight divided into 4 groups. The control group (subcutaneous injection of 0.1 ml physiological saline), subcutaneous epinephrine injection group 0.001 mg/20 g BW, subcutaneous epinephrine injection group 0.005 mg/20 g BW, the subcutaneous injection of epinephrine 0.01 mg/20 BB gr. Result: The results show that the analysis of spermatogenic cells (spermatogonia, spermatocytes, and spermatids oval), that repeated exposure to epinephrine with different doses showed that there was a signifi cant decrease. The average decline in a row in the treated group (P1, P2, P3) compared with the control group (K0) for spermatogonia was 28.8%, 42.4%, 45.5%, to spermatocytes was 13.7%, 37.4%, 38.9% and for oval spermatids was 33.3%, 44.2%, 50.4% Having followed by LSD 5% found that there were signifi cant differences for almost all groups except the pair P2–P3 groups for the number of spermatogonia, spermatocytes, and partner groups P1–P2 and P2–P3. As for the blood serum levels of testosterone hormone mice showed no signifi cant decrease. Discussion: Administration of high doses of epinephrine (as stressor) can lead to bottlenecks in the process of spermatogenesis as indicated by decreased number of spermatogenic cells but not cause a decrease in testosterone levels.
Authors and Affiliations
Yuni Sufyanti Arief
Keywords
Related Articles
- EP ID EP216079
- DOI 10.20473/jn.v6i2.3987
- Views 97
- Downloads 0
Demographical Factors, Not Lifestyle Factors, Associated with the Increase of Random Blood Glucose in Coastal Areas
Introduction: Blood glucose is an important component in the body produced by the consumption of carbohydrates, proteins, and fats. A blood glucose imbalance causes an increase in the blood glucose level in the body. The...
Effects of Health Education on Leptospirosis Prevention Through Dasawisma
Introduction: Globally, leptospirosis is still a major health problem in African and developing countries, including Indonesia. The best control effort is with prevention through health education. Health education with c...
Predictors of Mortality among Patients Lost to Follow up Antiretroviral Therapy
Introduction: The death of HIV/AIDS patients after receiving therapy in Bali is the seventh highest percentage of deaths in Indonesia. LTFU increases the risk of death in PLHA, given the saturation of people with HIV tak...
The Effect of Family Therapy with Spiritual Approach Toward Family’S Health Belief Model in Taking Care of Patient with Schizophrenia
Introduction: Schizophrenia is the problem with kognitive, mal-adaptive thought and behavior. Family who have a member with mental disorder can experience serious confl ict, become an objective and subjective burden, bla...
Nursing Intervention Through Family Pathnership Increases Behavior in Practice of Feeding Pattern on Infant of Age 6–24 Months for Nuclear and Extended Family
Introduction: Nursing intervention is nursing action with a supportive and educative approach done by nurses cooperating with families in overcoming the problems of nursing family. The aim of the research was to explain...