Structural Characteristics, Antitumor and Antioxidant Properties of Polysaccharides isolated from the brown algae Stypopodium schimperi growing on the Lebanese coast.
Journal Title: Advance Research Journal of Multidisciplinary Discoveries - Year 2017, Vol 17, Issue 9
Abstract
Objective: The purpose of this study was to extract, to determine the functional groups and to examine the antioxidant and anti-proliferative effect of the watersoluble polysaccharides fucoidan and sodium alginate extracted from the brown algae Stypopodium schimperi growing on the Lebanese coast. Materials and Methods: Fucoidan and sodium alginate were analyzed using 1H NMR, and FTIR methods to reveal their structures. Antioxidant effects were also tested, using DPPH method. The polysaccharides were tested in-vitro for anti-proliferative activity against the colorectal cancer cell lines (HT 29 and HCT 116) for 24h, 48h and 72h respectively, by the MTT cell viability assay. Results: The extraction yield of fucoidan and sodium alginate was 7.4% and 21% respectively. The structure of α-L fucose unit of fucoidan was confirmed through the spectrum of 1H NMR. M/G ratio of sodium alginate was calculated and found to be 0.96 which allows the formation of strong and heat stable gel. Both polysaccharides have an antioxidant effect with fucoidan the most pronounced. For the anti-proliferative effect, sodium alginate was able to inhibit the growth of HT 29 and HCT 116 more than fucoidan at the different time intervals. Conclusion: This study discusses the structure, the functional groups, the antioxidant and anti-proliferative effect of fucoidan and sodium alginate. Both polysaccharides showed an antioxidant effect which is dose dependent and they were able to inhibit the growth of HT 29 and HCT 116 with difference in the degree of inhibition between sodium alginate and fucoidan. These effects make them candidate in the field of medicine and food industry.
Authors and Affiliations
Haddad M, Kanaan H, Zein S, Karaki R, Krivoruchko E, Makhour Y, KASSEM Z
Keywords
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