Studied of CD14 and CD15 Gene Expressions for Myeloid Derived Suppressor Cell Profile at the RNA Level as a Predictor for Progressivity in Nasopharyngeal Carcinoma
Journal Title: Journal of Surgery - Year 2016, Vol 4, Issue 1
Abstract
Background: Nasopharyngeal carcinoma (NPC) is a disease in which malignant cells formed in the tissue of the nasopharynx, which is a frequent cancer in Indonesia. Estimated of NPC in Indonesia at 6.2/100.000 or about 12.000 new cases per year. Developments of tumors are influenced by many factors, generally an interaction between genetic, environmental and immune system. There is a role of myeloid derived suppressor cells in the process of immune suppression. Objective: The aim of this study is to clarify the profile of MDSC encoded by CD14 and CD15 genes expression to find the predictor for progressivity of nasopharyngeal carcinoma. Peripheral blood specimen and biopsy from primary tumor were collected from 16 nasopharyngeal carcinoma patients. The samples collected underwent qRT-PCR. Data were analyzed by 2-ΔΔCt methods and statistical analysis. Results: There were up regulation of CD14 and CD15 m-RNA level in blood at advanced stage (52.42 and 38.03) using 2-ΔΔCt methods. The expression of CD14 and CD15 genes was strongly correlated with clinical stage. Conclusion: MDSC can be used as a predictor of progression in NPC.
Authors and Affiliations
Dewi YA
Keywords
Related Articles
- EP ID EP203808
- DOI 10.13188/2332-4139.1000025
- Views 123
- Downloads 0
Postoperative Pneumoperitoneum: Clearing the Air
Introduction: Pneumoperitoneum seen on postoperative imaging presents a diagnostic dilemma. It can be a normal finding secondary to air that was introduced at surgery, which typically resolves in a matter of days. On the...
Interferon Treatment Improves Survival among Liver Transplant Recipients with Recurrent Hepatitis C
Hepatitis C in the liver allograft recipient has an aggressive course. A significant proportion of such recipients develop graft fibrosis and cirrhosis within five years of transplantation. Treatment efficacy with standa...
Invasive Procedures withQuestionable Indications used in Russia: Recent History
This review summarizes a series of reports on invasive procedures applied with questionable clinical indications. The following topics are discussed: the overuse of the Halsted and Patey mastectomy, relatively high negat...
Predictors of Solid Organ Injury Following Blunt Abdominal Trauma: A Single Institution Process Improvement Initiative
Background: Computed tomography (CT) scan remains the gold standard for identifying solid organ injuries (SOI) following blunt abdominal trauma but subjects the child to ionizing radiation, requires transportation out of...
Primary Thyroid Lymphoma: A Comprehensive Review of Contemporary Management
Primary thyroid lymphoma is an uncommon malignancy that constitutes fewer than 2% of all extranodal non-Hodgkin lymphomas (NHLs) and only 0.6% to 5% of all thyroid malignancies. Histologic subtypes include diffuse large...