Studies of heparine solutions preserved in glass and plastic ampoules by the metod of laser desorption mass spectrometry
Journal Title: Фармацевтичний журнал - Year 2014, Vol 4, Issue 4
Abstract
In this study the practical problem of permissibility of heparin preservation in ampoules made of various materials was investigated by methods of matrix-assisted and matrix-free laser desorption/ionization combined with time-of flight analyzer. It was shown that in the case of investigation of fabricated solutions of heparin by MALDI ToF method the investigation of heparin is restricted by presence of protein-stabilizer. Thus, the method of choice for investigation of heparin preservation in ampoules is the matrix-free laser desorption mass spectrometry. It was found out that heparin degradation takes place during the period of preservation in opened ampoules which fact is confirmed by increase of fragments number of structural units of heparin and this process is characteristic both for glass and plastic ampoules during the whole period of measurements. It has been also shown that, according to results of mass spectrometric analysis (in particular to character of obtained mass spectra and number of desorption products) the evolution of heparin from the surface of glass and plastic ampoules was almost similar during 14 days. Thus, plastic may be used as alternative to glass as material for fabrication of ampoules.
Authors and Affiliations
O. V. Severinovskaya, N. I. Karandeyeva, I. N. Osipova, A. G. Lisitskiy
Keywords
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