Studies of sediments, dry mud and water samples from lake elmenteita reveal the presence of thaumarchaeota and euryarchaeota
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 6, Issue 6
Abstract
So as to provide new insight into the diversity of Archaea in dry mud, sediment and water from Lake Elmenteita in Kenya, an investigation using a culture-independent approach was conducted after extraction of total genomic DNA from the environmental samples using suitable extraction methods. Small insert clone libraries were constructed by amplifying 16S rRNA genes using archaea-specific primers, followed by cloning from which 94 non-chimeric sequences were obtained and a total of 34 operational taxonomic units (OTUs) were identified. The OTUs were grouped into Thaumarchaeota (6%) and Euryarchaeota (94%). Approximately 90% of the clones were related with genes from uncultured Archaea, compared to the 10% that showed affiliation with genes from previously cultured Archaea. The similarity of the sequenced clones to type strains was consistently lower than the similarity to uncultured members of Archaea. Clones from water and sediment were dominated by sequences from Euryarchaea, while clones from the dry mud samples showed affiliations to both Euryarchaeota and Thaumarchaeota, with the predominant phylum being Euryarchaeota. This is the first study reporting the presence of representatives of Thaumarachaeota from Lake Elmenteita and adds sequences from Lake Elmenteita to the developing database of 16S rRNA clone libraries obtained from environmental sources.
Authors and Affiliations
Jacqueline O. Akanga, Hamadi I. Boga, Hans-Peter Klenk
Keywords
Related Articles
- EP ID EP38487
- DOI -
- Views 156
- Downloads 0
Study of the behavior/adaptation of non-target biological models exposed to multiple pollution
In this study, we were interested in assessing the impact of leachate leaves three plant species (Acer monspessulanum, Populus nigra, Salix babylonica) taken near petrochemical complex (Skikda N-E. Algeria) on Helix as...
Participatory Development of Soil Conservation Measures at the Debre-Mewi Watershed in the Upper Catchment of the Blue Nile Basin, Northwest Ethiopia
This study was carried out to assess the magnitude of soil erosion by water under different management practices [Fanyajuu with elephant grass (FEG), Fanyajuu with vetiver grass (FVG), sole Fanyajuu (SF) and non-conserv...
Removal of Cu(II) from aqueous solutions using dried activated sludge and dried activated nano-sludge: Adsorption isotherm and kinetics
The aim of this study was to investigate the effectiveness of dried activated sludge (DAS) and dried activated nano-sludge (DANS) for removal of Cu(II). Dried activated sludge and dried activated nano-sludge prepared as...
The morphology, anatomy and physiology characterization of mutant wheat (Triticum aestivum L.) “Alibey” in tropical lowland area
The performance information necessary to determine mutant wheat mutant traits in the breeding program of activities to increase production. The aim of this research was to obtain variability that could be used as select...
Intra species association of some yield parameters in half-sib families of Allium cepa L.
Allium cepa L. is considered as one of the most widely consumed bulb vegetable in topical and sub-tropical regions of the world, because of its flavour, in addition to other dietic and medicinal values. Poor onion genot...