Studies on Calli Mediated Multiple Shoot Regeneration from Rumex vesicarius L.
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 5
Abstract
Rumex vesicarius L is a valuable potent medicinal herb, which belongs to family Polygonaceae. It possesses antimicrobial, anti- inflammatory, antidiarrhoeal and antioxidant properties. An attempt to study the In vitro callus induction and regeneration of plantlets from calli of leaf and nodal segments as explants has been achieved. Initially mature seeds were excised from plants grown in the departmental garden of KL University. The sterilized seed explants were inoculated aseptically to the solid basal SH media without any growth regulators for seed germination. Effective plantlets observed after 1 week of culture inoculation under maintained controlled conditions. From these In vitro plantlets, leaf and nodal segments were taken as explants for this study. These explants were inoculated on SH medium supplemented with different concentrations of BA (0.5-5.0mg/l) and 2, 4-D (0.5-3.0mg/l) for callus induction and multiple shoot regeneration. 90% of callus induction was observed on media containing BA 4.0mg/l and efficient multiple shoot induction (96.6%) was observed on media containing BA 1.0mg/l from leaf explants. 90% of callus induction and 85% of multiple shoot induction observed on media containing BA 2.0mg/l from nodal explants. Roots were induced from In vitro shoots on SH medium supplemented with 1mg/l IBA after 1 week. Leaf explants were more regenerative with 96.6% response compared to nodal explants 85%. Finally these In vitro regenerated plantlets were hardened, acclimatized and successfully transferred to the field. This protocol will be useful for mass multiplication of plantlets and maintenance of germplasm throughout the year.
Authors and Affiliations
Chakravarthy Rama, Lavanya Kakarla, Mahendran Botlagunta, M. S. R Krishna
Keywords
Related Articles
- EP ID EP237314
- DOI 10.9734/BBJ/2014/8375
- Views 89
- Downloads 0
Antioxidant Properties of Fermented Kolanut husk and Testa of Three Species of Kolanut: Cola acuminata, Cola nitida and Cola verticillata
Excessive free radicals in the body system are known to cause oxidative stress, resulting in some pathological conditions which are fast becoming a challenge that needs urgent attention. Plants are known to be good sourc...
Phytochemical, Anti-nutrients and Toxicity Evaluation of Cleome gynandra and Solanum nigrum: Common Indigenous Vegetables in Zimbabwe
Aim: This study assesses the phytochemical profiles, toxicity and anti-nutrient properties of two indigenous vegetables, Cleome gynandra and Solanum nigrum. Methodology: Aerial parts of S. nigrum and C. gynandra collecte...
Proteomic Quantitative UV Absorption Spectrum Analysis of Effect of Heat Stress on Protein Extract from Cowpea Seed (Vigna unguiculata (L) Walp)
Aim: Proteomic quantitative UV absorption spectrum analysis was used to study the effect of heat stress on protein extract from cowpea seeds (Vigna unguiculata (L) Walp). Study Design: Protein extracts were obtained fro...
Molecular Diversity Analysis of Coat Protein Gene Encoded by Legume Begomoviruses and PCR Assay to Detect Yellow Mosaic Viruses Infecting Soybean in India
Aim: Coat protein (CP) genes encoded by Legume yellow mosaic viruses (LYMVs) were analysed to study molecular diversity and to devise effective PCR based assay to distinguish major Begomovirus species (Mungbean yellow mo...
Stability Evaluation and Degradation Kinetics of Ascorbic Acid in Baobab Fruit Pulp Formulated with the Seed Oil
Aims: Baobab (Adansonia digitata) fruit pulp and the seed oil contain appreciable amount of vitamins and nutrients which help fight off diseases and afford commendable source of nourishment. It is essential to screen for...