Studies on Growth Kinetics of the FMDV Serotype SAT-2 Egyptian Strain in Cell Culture
Journal Title: Journal of Veterinary Advances - Year 2013, Vol 3, Issue 2
Abstract
Foot and mouth disease virus (FMDV) Type SAT-2/Egypt/2012 isolated from El-Gharbiya Governorate was propagated six serial passages into monolayer BHK21 cell line. Growth curve of the infected virus, development of complement fixing (CF) antigens and 146s content in infected BHK21 cells incubated at 2 different temperature (35ºC and 37ºC) were studied using the 6th virus passage. Maximum infectivity titer with 100% CPE of the infected cells was detected 21 hours post inoculation at both incubation temperatures. The highest complement fixing antigens titer and 146S protein content of the same strain was recorded 18 hours post infection at both different incubation temperatures. So the best time of choice for virus harvest is 18hrs. post inoculation at 37ºC incubation.
Authors and Affiliations
Ali S. M. , Ismail A. H. , Soliman E. M. , Hanaa A. M.
Keywords
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