Studies on Immobilization of Cutinases from Thermobifida fusca on Glutaraldehyde Activated Chitosan Beads
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 10
Abstract
Aims: To evaluate and optimize the activity and stability performance of two recombinant cutinases of Thermobifida fusca, Cut1 and Cut2 on glutaraldehyde activated chitosan beads. Place and Duration of Study: Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Assam India. Experiment conducted as a partial fulfillment to PhD degree from December, 2010 to January, 2014. Methodology: Purified cutinase were immobilized on chitosan beads by covalently coupling with glutaraldehyde. The biophysical properties of immobilized cutinase was analysed by FTIR, FESEM and the operational stability and activity of the immobilized cutinase was studied at different pH and temperature. Results: The optimal immobilization was achieved with 3% (v/v) glutaraldehyde activation and coupling pH of 8.5. Under this condition, 74% and 71% of immobilization was achieved for Cut1 and Cut2, respectively. Immobilized cutinase showed optimal activity at pH 8 with optimal functional range of pH 7.5 to 9 and 55ºC with operational stability in the range of 45ºC to 70ºC. The reusability and storage stability was found to be 80% after 10 reuse cycles and 50% after 13 days, respectively as compared to its initial activity. There was no loss in activity even after repeated freeze drying. Conclusion: the cutinase immobilized on glutaraldehyde activated chitosan beads demonstrated better operational stability in comparison to free cutinase, showing chitosan as a potential support in the enzyme immobilization technology for industrial applications of T. fusca cutinases.
Authors and Affiliations
Venkata Dasu Veeranki, Krishnamoorthy Hegde
Keywords
Related Articles
- EP ID EP237736
- DOI 10.9734/BBJ/2014/12558
- Views 96
- Downloads 0
Influence of Various Nitrogen Sources on Biomass and Lipid Production by Chlorella vulgaris
Chlorella vulgaris is a unicellular, photosynthetic fresh water green alga with high concentration of chlorophyll. This microalga synthesizes biomass by trapping energy from the sun. It has valuable components particular...
Anti-tumor Effect of Calloselasma rhodostoma Venom on Human Breast Cancer Cell Line
Aim: Breast cancer is a major health issue for women worldwide. The potential anti-tumor effect of snake venom, has been studied and evidences showed reducing tumor size and inhibition of angiogenesis. Present study aims...
Screening and Media Optimization for Enhancing L-asparaginase Production, an Anticancer Agent, from Different Filamentous Fungi in Solid State Fermentation
Aim: The aim of present study was to screen new potent fungal isolates and microorganisms possessing extracellular L-asparaginase production capacity. In addition, optimization of cultural and environmental conditions re...
Production of Some Polysaccharides from Kojic Acid Producing Aspergillus oryzae var. effusus NRC14 Biomass
Aims: The present investigation describes the utilization of Aspergillus oryzae var. effusus NRC14 biomass producing kojic acid in the production of fine chemicals that have valuable applications such as chitin, chitosan...
Inhibitory Effects of 4T1 Breast Tumor Transplantation on Mouse Peripheral Blood Immune Cell Populations
Aims: One of serious threats to women's health is mammary cancer whose occurrence, development, and treatment are related to the body’s immunological circumstances. In addition, the cancer also imposes the some effects o...