STUDIES ON MICROPROPOGATION IN VITRO SHOOT INDUCTION FROM STEM NODE EXPLANTS OF CUCUMIS SATIVUS L.
Journal Title: IJAR-Indian Journal of Applied Research - Year 2019, Vol 9, Issue 11
Abstract
Tissue culture techniques are now becoming popular as alternative means of vegetative propagation. The effect of Benzyl amino purine in inducing shoot induction was already reported in some of the important medicinal plants (Komalavalli and Rao, 2000). The stem node explants were inoculated on MS medium supplemented with various cytokinins i.e., BAP and NAA. Addition of BAP at 3.0 mg/l concentration or NAA at 3.0 mg/l to the MS basal medium, induced regeneration from the stem node explants. Micro propagation involves multiplication of genetically identical individual by asexual reproduction within a short span of time with tremendous potential for the production of high quality plant based medicines (Murch et al., 2000). Multiple shoot induction was achieved in one of the important medicinal plant of Cucurbitacae family, Cucumis Sativus. MS medium supplemented with 1.0 mg/l BAP + 3.0 mg/l NAA and 2.0 mg/l L-Glutamicacid was found to be optimum to induce shoots. The present study established reliable and reproducible protocol for rapid multiple shoot induction from stem node explants of Cucumis Sativus using different concentrations and combinations of Cytokinins.
Authors and Affiliations
Mandaloju Venkateshwarlu*
Keywords
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