STUDIES ON MOLECULAR CHARACTERIZATION OF RAGI BLAST PATHOGENS FOR VARIABILITY AMONG PYRICULARIA GRISEA (CKE) SACC. ISOLATED FROM VARIOUS REGIONS OF TAMIL NADU BY RAPD MARKERS ANALYSIS
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 14
Abstract
Pyricularia grisea (Cke) Sacc incites blast disease in finger millet and rice. An r-DNA region was amplified from the 12 isolates of P. grisea (B1, B2, B3, B4, B5, B6, B7, B8, B9, B10, B11 and B12) isolated from finger millet and rice, using the universal primers to form a phylogenetic tree revealed that the isolates fall in 3 different clusters. Where in, ITS1-5.8S – ITS5 sequences and Randomly Amplified Polymorphic DNA profiling of P.grisea isolates used in the study clustered into two main groups, in which the isolates B1, B2, B3, B4, B5, B6, B7, B8, B9, B10 and B11 formed one group with approximately 12 per cent similarity coefficient between them and B12 was grouped in separate cluster. Molecular characterization of ragi blast pathogens in the study by RAPD markers analysis segregated the isolates into different clusters confirmed that the rice and finger millet-infecting P. grisea populations of India are clearly distinct.
Authors and Affiliations
ARUNKUMAR NAGARATHINAM
Keywords
Related Articles
- EP ID EP170505
- DOI -
- Views 93
- Downloads 0
LIVESTOCK OWNERS’ PERCEPTION TOWARDS WILDLIFE CONFLICT: A METHODOLOGICAL APPROACH
Conflict between livestock owners and wildlife are reported to be increasing over the time that leads to negative consequences on local communities in the vicinity of protected areas. The shift in conservation resulted i...
EFFECT OF FOLIAR SPRAY OF UREA AND ZINC SULPHATE ON MORPHOLOGICAL, YIELD AND QUALITY ATTRIBUTES OF GUAVA (Psidium guajava L.) Cv. APPLE COLOUR
An experiment was conducted on seven year old tree of guava cv. Apple Colour at Mandsaur, (M.P.) to study the effect of nutrients spray on morphological, yield and quality attributes of guava. The plant height increased...
EFFECT OF HERBICIDAL APPLICATION ON INCIDENCE OF INSECT PESTS AND NATURAL ENEMIES
The incidence / population of various insect pests and natural enemies were recorded at weekly interval on rice cultivar CSR 30 under transplanted condition with different herbicidal treatments during Kharif 2013 and 201...
IMPACT OF AVAILABLE N, P2O5 and K2O ON SOIL DUE TO DIFFERENT MANAGEMENT PRACTICES AFTER GROWING SALI RICE
A field experiment was conducted at Instructional-cum-Research (ICR) farm, Assam Agricultural University, Jorhat-13, Assam during kharif 2015. The experiment was laid out in split-plot design replicated thrice with four...
EFFECT OF DIFFERENT MULCHES ON GROWTH, YIELD AND ITS ATTRIBUTING CHARACTERS OF CHILLI (Capsicum annuum L.) CV. KALIPEETH
The present field investigation in chilli resulted the treatment T2 (hand weeding at the interval of 15 days) was recorded maximum growth and yield viz., plant height, number of branches per plant, days of first flowerin...