STUDIES ON PHENOTYPIC TRAITS AND GENETIC VARIABILITY IN ERG 11 GENE AMONG DRUG RESISTANT CANDIDA SPECIES
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2013, Vol 1, Issue 9
Abstract
Candida is an opportunistic pathogen of human beings and other mammals. It is the leading agent of various types of diseases in immunocompromised individuals. The most common infecting species is C. albicans where as other non albicans species are C. glabrata, C. parapsilosis, C. tropicalis and C. krusei also causes various clinical diseases. Candidemia is the fourth leading cause of bloodstream infections and carries a 35–55% mortality. The widespread use of azoles has led to increasing azole resistance among Candida albicans strains. One mechanism of azole resistance involves point mutations in the ERG11 gene, which encodes the target enzyme (cytochrome P450 lanosterol 14α-demethylase). Aim of study was to isolate azole-drug resistant Candida species and to observe genetic variability in ERG11 gene, also phenotypic characterization of different Candida species. In the present study, 24 strains were examined for phenotypic characters such as the pseudohyphae, chlamydospore formation and germ tube production and some colored colonies on chrome agar. Antifungal susceptibility testing was also done by placing fluconazole and voriconazole disk on Muller Hinton agar. Zone of inhibition of various diameters were formed around these disks by which we identified resistant and sensitive Candida sp. Two isolates of Candida tropicalis were resistant to both the antifungal agents. The ERG 11 gene of two azole drug resistant isolates was then amplified using forward and reverse primers. The two drug resistant amplified products were then sequenced and the sequence results were compared with homologous species.
Authors and Affiliations
Surabhi Kaura*| Departement of Microbiology, Shoolini Institute of Life Science and Business Management, Solan, India, Surjeet Singh| Departement of Microbiology, Shoolini Institute of Life Science and Business Management, Solan, India, Amit Kumar| Departement of Microbiology, Shoolini Institute of Life Science and Business Management, Solan, India
Keywords
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