Study of extraction efficiency of prenylated flavonoids from hops raw (Humulus lupulus L.) by the ethanol containing extraction solvents
Journal Title: Фармацевтичний журнал - Year 2015, Vol 2, Issue 2
Abstract
Development of technology of the hop dry extract as APIs provided for a detailed study and identification of factors that can affect the quality characteristics of the finished product. The goal of this study was to investigate the effect of ethanol concentration in the water alcohol extraction solvent on the extraction efficiency of prenylated flavonoids from the thermal pretreated and fat-free hop cones. The investigated samples of extracts obtained according to the general process scheme which included the following steps: thermal isomerization of prenylated chalcones to corresponding prenylated flavanones, removal of lipophilic compounds from thermal pre-treated raw material by n-hexane extraction, extraction of the defatted raw material by the ethanol containing extraction solvent, evaporation and drying of water-ethanol extract in vacuum. Extraction steps were performed using filtration extraction method. As extraction solvents on the second extraction step were 50%, 70% and 90% (vol.) ethanol solutions. Efficacy of water-ethanol extraction solvents was evaluated by the yield of extractable matter, yield and quantitative content of the xanthohumol (X), isoxanthohumol (IX), 8-prenylnaringenin (8-PN) and 6-prenylnaringenin (6-PN) in the obtained dry extracts. The HPLC method was used for assay. According to the study 70% (vol.) ethanol showed the highest efficiency, its extraction ability was evaluated by the highest yield of 8-PN (0,023%) and yield of extractable matter (25,71%) as well as aggregate content of xanthohumol (2,226%), isoxanthohumol (0,405%), 8-prenylnaringenin (0,091%) and 6-prenylnaringenin (0,261%) in the dry extract.
Authors and Affiliations
O. O. Dobrovolnyi, O. P. Shmatenko, A. S. Shalamay, V. V. Rudenko
Keywords
Related Articles
- EP ID EP583317
- DOI -
- Views 37
- Downloads 0
The use of integrated frequency ABC/VEN-analysis of application of medicines for the treatment of influenza and its complications in the hospital
The problem of limiting and rational use of funds in the health care system is relevant worldwide. Influenza and acute respiratory viral infections are cause of a lot of discussion about the treatment and prevention of t...
Isolation gidazepam and its metabolites by solid-phase extraction
Gidazepam as benzodiazepine derivative is drugs of abuse and is object of toxicological research. The first phases of analysis of analite is its insulating from biological objects. In a case of gidazepam such analites is...
Determination of the sum of flavonoids in hops (Humulus L.) with the use of luminescent sensor on the basis of the complex of rutin with yttrium (III)
Hop polyphenols are one of the main groups of biologically active agents defining medicinal properties of hops. Various chromatographic, electrochemical, сhromatographic methods of the analysis are used for the determina...
Dynamics of prostate protectors assortment in the state formulary of medicines of 1-9 issues
Prostatitis is one of the most frequent urology diseases of men of reproductive age. Recommendations for rational pharmacotherapy prostatitis are included into the State Formulary of Medicines, which is reviewed and upda...
Historical genesis in establishment of pharmaceutical business in Ukraine in an diferens stages of its social-economic and political development. P a r t I. Period 1917–1930
Positive experience in organization of providing the population with medicine that has great scientific and practical interest for contemporaries was accumulated by previous generations of pharmacists. The aim of the st...