Study of oxidative damage by myeloperoxidase derived reactive oxygen
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 2
Abstract
Neutrophils enhance the production of reactive oxygen as a result of the priming reaction by various cytokines. However, the relationship is still unknown between the priming reaction and releasing of myeloperoxidase (MPO). Furthermore, the oxidized stress disorder has not been fully studied yet. We examined the activation of neutrophils by various stimulating substances, MPO releasing activity by the priming reaction of TNF-α, and the oxidation disorder from the MPO-derived reactive oxygen. The study found that priming by TNF-α with f-MLP stimulation enhanced the production of reactive oxygen. The releasing of MPO increased only with f-MLP stimulation, and the priming by TNF-α increased the releasing of MPO even more. The reactive oxygen species derived by the releasing of MPO enhanced the lipoperoxidative reactions and cellular membrane defect. The above results indicate that MPO-derived reactive oxygen species increase cellular disorder and are related to a clinical condition with oxidized stress.
Authors and Affiliations
Yasuhiko Ohta
Keywords
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