Study Of Phytohormone Autonomy In In Vitro In Petunia Hybrida

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Study Of Phytohormone Autonomy In In Vitro In Petunia Hybrida

Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 2

Abstract

ABSTRACT Petunia x hybrida is used as noble genetic material for somatic hybridization, molecular studies and hence is of concern in phylogenetic significance to serve as a useful models of physiology and host - parasite interaction. In the present study using two variants of Petunia hybrida, the phytohormone autonomy on different formulation of MS medium was carried out. The type of response on hormone free MS medium was rhizogenesis in both the variants. When hormones IAA was substituted, the results of phytohormone independent growth in culture was a different in purple and white variants. Molecular level investigation for the role of Agrobacterium rhizogenes rol A,B,C genes in rhizogenesis and autonomous behavior is confirmed with specific amplification pattern. Using the combination of cyclin GTcyc gene isolated from tumorous hybrid GGLL ( N.glauca x N. langsdorfii) and D3 cyclin primers, the cytogenetic behavior is known to be a cumulative effect of activation of these genes at molecular level.

Authors and Affiliations

S. Sharmila Begum1* and A. Arundhati2

Keywords

EP ID EP101773
DOI -
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