Study of synergy effect of benzalconium chloride on antimicrobial activity of vegetable essential oils
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. In the modern world it is important to create new more natural and biodegradable substances for the sanitation and disinfection of livestock buildings, especially in the presence of animals. The goal of the work was to study the minimum effective concentrations of plant essential oils toward test microorganisms and to reveal the synergistic effect of benzalkonium chloride on antimicrobial activity. Materials and methods. To detect the antimicrobial activity of essential oils, the following test cultures were used: E. coli, B. cereus ATCC6633, S. aureus 209-P, Pasteurella multocida, which are stored in the Museum of the Institute of Veterinary Medicine. The research was carried out using methods modified for the essential oils insoluble in water: macrotests in a nutrient medium, followed by cultivation under conditions that are optimal for microorganisms; the method of diffusion into agar using paper disks, impregnated with certain concentrations of essential oils. Essential oils were used in the studies: fir, eucalyptus, sage and thyme. To evaluate the activity of antibiotic substances and their minimally effective concentrations macro and micro methods were used and the method of diffusion in agar. Results of research and discussion. The most effective against test microorganisms – mixtures of Staphylococcus aureus 209-P, Pasteurella multocida 115, E. coli 1257, B. cereus ATCC6633 cultures were essential oils of thyme and sage, while eucalyptus and fir showed less activity. As a result of the studies, a composition has been chosen that provide a stable aqueous suspension containing two plant essential oils that have solid antimicrobial properties, which can be further diluted with water to obtain the required working concentration of the preparation. The antimicrobial effect is enhanced by the addition of quaternary ammonium compounds – benzalkonium chloride, which provides a synergistic antimicrobial effect. We have experimentally confirmed the synergistic effect of benzalkonium chloride on the antimicrobial activity of preparations based on plant essential oils, especially thyme oil (inhibition of growth of log 5,0±0,22), eucalyptus (log 4,9±0,20), sage (log 2,6±0,31) and fir (log 4,7±0,13). Other combinations also showed quite a high activity compared to the activity of plant essential oils of thyme, fir, eucalyptus and benzalkonium chloride. Conclusions and prospects for further research. In experiments with test microorganisms Staphylococcus aureus, Pasteurella multocida, E. coli, Bacillus cereus was confirmed the antibiotic effect of the essential oils of thyme, sage, eucalyptus and fir, and confirmed the possibility of its enhancement by the addition of quaternary ammonium compounds. The synergistic effect of benzalkonium chloride on the antimicrobial activity of preparations based on vegetable essential oils, especially thyme oil (growth inhibition log 5,0±0,22), eucalyptus (log 4,9±0,20), sage (log 5,6±0,17) and fir (log 4,7±0,13). Preparations that are planned to be further developed on the basis of synergistic interaction of antibiotics, vegetable essential oils and their complexes will be used for the treatment of livestock farms.
Authors and Affiliations
Oleksandr Tarasov, Basil Sapeyko, Olena Aishpur, Maria Babkina, Svitlana Tereshchenko, Iryna Zotsenko
Keywords
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- EP ID EP406279
- DOI 10.31073/vet_biotech32(2)-64
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