Study on alantolactone-induced differentiation of mesenchymal stem cells into vascular cells

Journal Title: Traditional Medicine Research - Year 2018, Vol 3, Issue 4

Abstract

To promote efficient screening of active angiogenic drugs from traditional medicines, we constructed a human embryonic kidney-293 cell model using vascular endothelial growth factor (VEGF) gene promoter as the drug target. In this model, VEGF gene promoter may regulate the expression of the luciferase reporter gene by responding to the stimulation of drug molecules. This cell model allows rapid and efficient screening of vascular-inducing active components from several drug monomer molecules. Furthermore, we used rat bone marrow mesenchymal stem cells (rMSCs) to conduct a preliminary study on the activity of alantolactone. Using simvastatin as a positive control, we investigated the effects of alantolactone on the expression of vascular-related cell marker molecules such as VEGF and α-smooth muscle actin (α-SMA) in rMSCs. According to our results, 0.1, 1, 3 and 5 μM of alantolactone upregulated the transcriptional luciferase gene activity of VEGF promoter, and a significant difference from that in the control group was observed. Among them, 3μM of alantolactone showed the better effect than that of 3 μM of simvastatin (P = 0.036) and other concentrations of alantolactone and simvastatin showed similar effects. Compared with that in the control group, rMSCs induced with 1μM alantolactone for 3 days showed a significant increase in the relative mRNA expressions of VEGF and α-SMA genes. However, these effect of 5 μM alantolactone were weaker than those of 5 μM simvastatin (P < 0.05); rMSCs treated with 1 μM alantolactone for 3 days showed brighter green fluorescence (FITC marker) of α-SMA and VEGF in situ expression than that observed in the control group and similar fluorescence intensity than that of simvastatin group in an immunoradiometric assay. The above results demonstrate the reliability of the highly efficient system for screening of active drug molecules and confirmed the vascular induction function of alantolactone at the gene and protein levels.

Authors and Affiliations

Yan-Jiao Lu, Qiong Lu, Ruo-Ke Su, Gang Wang, Rui Tan

Keywords

Related Articles

Fireneedle up-regulates the expression of ERK1/2 in neural stem cells

Objective: To explore the effect of fire needle on the expression of ERK1/2 of neural stem cells (NSCs) obtained from rats with spinal cord injury (SCI). Methods: Sixty SD female rats were randomly divided into 5 groups,...

A new strategy of enhancing absorptivity and safety for evodiamine: polyamidoamine derivatives modified by polyethylene glycol chain

Background: Traditional Chinese medicine involves complex ingredients and mixtures of ingredients that often exhibit low bioavailability, and excipients are often lacking to increase the absorption-enhancing effects. Thi...

Continuous ban on injections of traditional Chinese medicines

Recently, the State Drug Administration of the People's Republic of China issued a series of notifications to ban certain herbal injections or modify the instructions for their use: May 29, 2018: A contraindication was...

Investigation of in vitro antioxidant activity of dihydromyricetin and flavonoids rich extract from vine tea (Ampelopsis grossedentata)

Background: Vine tea from fermented Ampelopsis grossedentata leaves has been used as a herbal tea and folk medicine in the southern region of China for hundreds of years. The aim of this investigation was to analyze the...

Advances in traditional Chinese medicine for cardiovascular disease therapy in 2020

Cardiovascular diseases are a major cause of morbidity and mortality worldwide and there is an urgent need to develop new pharmacotherapies for managing cardiovascular diseases. In China, traditional Chinese medicine has...

Download PDF file
  • EP ID EP45226
  • DOI 10.12032/TMR201812077
  • Views 282
  • Downloads 0

How To Cite

Yan-Jiao Lu, Qiong Lu, Ruo-Ke Su, Gang Wang, Rui Tan (2018). Study on alantolactone-induced differentiation of mesenchymal stem cells into vascular cells. Traditional Medicine Research, 3(4), -. https://europub.co.uk/articles/-A-45226