STUDY ON ANTIMYCOBACTERIAL ACTIVITY OF MARINE ACTINOMYCETES FROM COROMONDAL COASTAL REGION OF SOUTHERN INDIA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: The objective of the present study is to screen the novel actinomycetes from marine sediment active against mycobacterium sp.Methods: actinomycetes were isolated by crowded plate technique and its antimycobacterial activity was determined by stroke method followed by optimized fermentation. The active fraction was extracted and bio assayed by TLC.Results: Totally 25 actinomycetes were isolated from 9 Marine sediment samples and frequency of isolates were, 40% of Streptomyces sp (ECRST) 32% of Micromonospora(ECRMM), 16% of Micropolyspora (ECRMP) and 12% were Streptoverticillium (ECRSV). Among the 25 isolates Microployspora a rare actinobacteria designated as ECRMP 4 showed potent antimycobacterial activity against M.tuberculosis. The zone of inhibition was 20 ± 0.1mm and the percentage of relative inhibitory zone was 62 against M.tuberculosis. The bioassay of crude compound prevails that the Rf value of compound is 0.16.Conclusion: The present study concludes that the isolated marine Micropolyspora capable to produce noval antimycobacterial compound effective against M.tuberculosis.
Authors and Affiliations
Gaja Lakshmi, A. Raja, R. Arthur James
Keywords
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