STUDY ON ANTIOXIDANT AND CYTOTOXIC PROPERTIES OF OLEA DIOICA ROXB. CRUDE EXTRACT AND ITS PURE COMPOUND COLLECTED FROM WESTERN GHATS, KARNATAKA, INDIA.
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 2
Abstract
Introduction: Olea dioica Roxb. an important medicinal tree plants used by local siddha tribes, belongs to the family Oleaceae. The parts such asleaves, bark, root, and fruits used in the traditional medicine to cure skin diseases, rheumatism, fever, and cancer.Objectives: The anti-oxidant experiment by metal chelating activity, superoxide radicals, hydroxyl radical, 2,2-diphenyl-2-picrylhydrazyl radicals,2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid radical scavenging assays with in vitro cytotoxicity was tested using trypan blue dye exclusiontechnique and 3-(4, 5 dimethylthiazole-2yl)-2, 5-diphenyltetrazolium bromide assay was conducted.Results: Anti-oxidant experiments revealed that the bark ethanolic extract of the O. dioica plant parts has excellent radical scavenging activity and itsextracted pure compound, Benzene ethanol, 4-hydroxy-alcohol, showed excellent radical scavenging activity higher than the standards used. In vitrocytotoxicity experiments revealed that bark ethanolic extract has excellent cytotoxicity activity and its pure compound benzene-ethanol, 4-hydroxyalcoholalso showed excellentactivitywhichis comparablewith the standardcurcumin.Conclusion: O. dioica bark could be exploited as a valuable source of antioxidant and cytotoxic agent for pharmaceutical industry.Keywords: Olea dioica Roxb, Metal chelating, Superoxide radicals, Hydroxyl radical, 2,2-diphenyl-2-picrylhydrazyl radicals, 2,2-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid radical scavenging,Trypan blue, 3-(4,5dimethylthiazole-2yl)-2,5-diphenyltetrazolium bromide assay, Benzeneethanol, 4-hydroxy-alcohol.
Authors and Affiliations
Ashwathanarayana Rao
Keywords
Related Articles
- EP ID EP605469
- DOI 10.22159/ajpcr.2017.v10i2.15727
- Views 70
- Downloads 0
DEVELOPMENT OF AQUEOUS POLYMERIC DISPERSION (APD) OF EUDRAGIT E PO- PERFORMANCE CHARACTERIZATION FOR AQUEOUS BASED TASTE MASKING COATING SYSTEM
The present research work was performed to develop an Aqueous Polymeric Dispersions (APD) of Eudragit E PO using emulsification solvent evaporation technology including optimization of solvent, emulsifier & stabilize...
EXCLUSIVE BREASTFEEDING, COMPLEMENTARY FEEDING, LOW BIRTHWEIGHT, AND WASTING IN CHILDREN UNDER-FIVE: A PATH ANALYSIS EVIDENCE FROM INDONESIA
Objective: Studies using life course perspective, longitudinal data, and path analysis on the relationships between exclusive breastfeeding (EBF), complementary feeding (CF), low birthweight (LBW), and wasting are lackin...
SURVEY, ISOLATION, BIOCHEMICAL CHARACTERIZATION, AND IDENTIFICATION OF HELICOBACTER PYLORI FROM GASTRIC PATIENT BIOPSY
Objective: This study was to isolate and identify the Helicobacter pylori from the biopsy samples of the gastric patient.Methods: Gastric biopsies were collected from the antral region of the gastric patient. Out of 96 p...
DRUG UTILIZATION PATTERN OF ANTIDIABETIC DRUGS AMONG DIABETIC OUTPATIENTS IN A TERTIARY CARE HOSPITAL
 Objective: The aim was to evaluate the drug utilization pattern of anti-diabetic drugs in diabetic outpatients and monitor the adverse drug reactions(ADRs) associated with anti-diabetic therapy.Methods: A prospective o...
DEVELOPMENT AND CHARACTERIZATION OF NEVIRAPINE LOADED AMORPHOUS SOLID DISPERSIONS FOR SOLUBILITY ENHANCEMENT
Objective: The present study entails the development of nevirapine (NVP)-loaded solid dispersions for improvement of solubility and in vitro profile. Methods: Solid dispersions were prepared through blending with a hydr...