Study on Citric Acid Production and Antibacterial Activity of Kombucha Green Tea Beverage during Production and Storage
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 16, Issue 3
Abstract
Aims: The aim of this study was to determine the amount of citric acid production and antibacterial activity of Kombucha green tea during its production and storage. Study Design: Experimental study. Place and Duration of Study: Department of Public Health, Maragheh University of Medical Sciences, between December 2016 and May 2017. Methodology: The amount of citric acid at two temperatures of 20°C and 30°C was determined using the HPLC technique during 21 days. To survey the antibacterial effect of Kombucha on the growth of Staphylococcus aureus, Salmonella typhimurium and Lactobacillus rhamnosus bacteria, two processes of well and the disc were used. Results: Production of citric acid undergone a change at 20°C from 5.92 on day 1 to approximately 31.75 on day 21, and this difference was significant. Additionally, the amount of this organic acid at 30°C changed from 5.57 on day 1 to 15.43 on day 21. The amount of citric acid produced during storage at 20°C was significantly greater than that at 30°C (p<0.05). In the well method, for Staphylococcus aureus and Salmonella typhimurium at both temperatures of 20°C and 30°C, the diameter of the formed halo between different days was significantly different (p<0.05). In the disc method, for Staphylococcus aureus at 20°C and 30°C the halo diameter in all experimental days were significantly greater (p<0.05) than day 1. For Lactobacillus rhamnosus bacteria, no halo was formed around the discs and wells. Conclusion: By time increase, the pH decreased the amount of citric acid increased, and the halo diameter around the well and disk (in all positive cases) increased.
Authors and Affiliations
Fereshteh Ansari, Hadi Pourjafar, Sahel Esmailpour
Keywords
Related Articles
- EP ID EP313381
- DOI 10.9734/ARRB/2017/35664
- Views 116
- Downloads 0
Effect of Flooding on Trypanosome Infection Rates in Trade Cattle at Central Abattoir, Makurdi Metropolis, Benue State, North Central Nigeria
Aim: The aim of the study was to determine trypanosome infection rates in cattle brought to Makurdi central abattoir during a devastating flooding of 2012. Study Design: Cattle of mixed sexes and breeds were randomly sam...
Effects of Routinely Oxytocin Injection to Induce Milk Ejection on Some Reproductive Parameters of Crossbred Cows in the Tropics of Veracruz
The objective was to assess the effects of routine intramuscular injection ofoxytocin in crossbred milked cows in days to first service (DPS), number of services per conception (SC), percentage of abortions (PA), cumulat...
A Century of Change in an Indonesian Coral Reef: Sluiter's Brandewijnsbaai (1890) Revisited
Corals of Terlena Island were first mentioned more than 125 years ago, when Sluiter in 1890 came up with a new theory of initial reef development. Our aim was to re-visit the reef, investigate the coral community and env...
Cognitive Function in Male Track and Field Iranian National Team Athletes
Background: Cognitive function including decision making and short term memory are affected by environmental factors including physical activity. Data showed that sustained physical activity can improve learning and memo...
Evaluation of Chemical Properties of Date-Palm Waste as Culture Media and Its Effect on Number and Yield of Tomato
Aims: The objective of this research was to evaluate the chemical properties of Date-Palm waste as culture media and its effect on number and yield of tomato fruit. Study Design: The experiment was conducted as factoria...