Study on Seed Mycoflora of Different Varieties of French Bean (Phaseolus Vulgaris L)
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 4
Abstract
Seeds of eight French bean varieties were selected to study the occurrence of seed mycoflora. Isolation was done by Direct Plate method (Warcup, 1950) and Dilution Plate method (Johnson and Curl, 1972) using Rose Bengal Agar medium. A total of 17 genera, 31 fungal species and 2 sterile mycelia were isolated from the seed varieties. The most abundant species were found to be species of Aspergillus, Cladosporium and Pencillium while the least abundant were species of Actinomucor, Eupenicillium, Fusarium, Nectria and Rhizopus. The highest colony form unit (CFU) was observed in FB 62 and FB 61 in Direct Plate method and Dilution Plate method respectively, while the lowest CFU was observed in Manipur variety in Direct Plate method and Naga Local variety in Dilution Plate method. From the present study, it was observed that a large number of fungal species colonized all the seed varieties studied which may cause deterioration in the seed quality and poor performance of the crop in the field. It was also observed that the Direct Plate method was more suitable for the isolation of seed microflora in comparison to Dilution Plate method.
Authors and Affiliations
Yarielyn Saio, Mamtaj Sahana Dkhar
Keywords
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