Study on the effectiveness of RIZOBAC in the process of calllogenesis in potato var. Desiree
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 4
Abstract
This experiment was carried out in a plant biotechnology laboratory at Havana Agronomy University (UNAH), evaluating the effects of different concentrations of bacterial preparations (RIZOBAC) on the obtention of fresh mass and on embryogenesis caracteristics of potato (Solanum tuberosum, L.) var. Desirée, callus in vitro obtained from the potato Germoplasm Bank of the Departament of Genetics and Plant Improvement, at the National Institute of Agricultural Science (INCA) as well as to estimate the possible substitution of plant growth regulators such as 2,4-D and kynetine in the culture media, to establish callogenesis of this crop. Different treatments were studied employing the established crop media as the control treatment; besides, the substitution of the auxins (3.0 mg.l-1) and citokinins (0.5 mg.l-1), with the bioactive complex (RIZOBAC) in different concentrations (0.5; 1.0; 1.5 y 2.0 mg.l-1), and in other cases adding to the control culture media, the same concentration. The fresh weight, colour changes, texture and consistence of the callus were evaluated. Variations were noted in the clone response as well as in the indicators. The best results were obteined using (1.5 mg.l-1) RIZOBAC as a sustitute for auxins. Favorable results were also obtained when the (RIZOBAC) at concentration of 1, 5 mg.l-1 was included in the original culture media that contained the two plant growth regulators. More than 10cts USD could be saved per litre of culture media with the substitution of 2, 4-D for the RIZOBAC.
Authors and Affiliations
Jaime Ramiro Hidrobo Luna, Eduardo Héctor Ardisana, Annia Hernández
Keywords
Related Articles
- EP ID EP697819
- DOI -
- Views 77
- Downloads 0
Use of biological agents for phytosanitary control in the production of potato minitubers under acclimatization conditions
The employment of biological controls of pests and diseases is a technology with trends to increase at present for being economic, lasting and of beneficial results, related to the stability that they offer the ecosyste...
Effect of pruning in biomass production and steviol glycosides contents of Stevia rebaudiana Bertoni var. Morita II
For the production of dry leaf of Stevia rebaudiana Bertoni under the Cuban climatic conditions, it is necessary to identify the agrotechnical variables with the greatest impact on its yield. Therefore, the objective of...
Methodology for the molecular diagnosis of Mosaic Virusof the Dasheen for the certification of in vitro plants of clones dasheen commercials
The quick propagation of Dasheen clones (Xanthosoma spp) and (Colocasia esculenta L.) through biotechnical techniques has generated a great demand of free of diseases lines, especially to the Dasheen Mosaic Virus, this...
Phenotypic variability in the field of Saccharum spp. hybrid cv. ‘C87- 51’plants regenerated via somatic embryogenesis
Sugarcane (Saccharum spp.) is a crop of great economic importance that has been propagated by tissue culture through organogenesis and somatic embryogenesis. The present research had as objective to determine the pheno...
Cytogenotoxicity potential of Syzygium aromaticum extract, an alternative treatment for Chenopodium quinoa seed
Chenopodium quinoa Willd (quinoa) is a pseudo cereal that has been gaining prominence in production in several regions, due to increased demand in the consumer market. In the search to reduce productivity losses caused b...