SUB-ACUTE ORAL TOXICITY PROFILING OF THE METHANOLIC LEAF EXTRACT OF CLINACANTHUS NUTANS IN MALE AND FEMALE ICR MICE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 12
Abstract
Objective: This study aims to investigate the sub-acute oral toxicity of a methanolic leaf extract of Clinacanthus nutans (MECN) in male and female mice. Methods: The study used three groups of male and female mice, the crude MECN was dissolved in distilled water and administered orally in single doses of 1000 and 2000 mg/kg body weight for 28 d by gavage, at a dosing volume of 10 ml/kg body weight, while the control group received only the distilled water. Mice were weighed weekly for the duration of the study period. After the treatment period, the blood samples were collected and examined for hematology changes, total red blood cell (RBC) and total white blood cell (WBC), packed cell volume (PCV) and plasma protein concentration. The serum was analyzed for liver and kidney function test. The degree of injury of the liver and kidney tissue was histopathologically assessed and scored under the light microscopy. one-way analysis of variance (ANOVA) with Turkey’s test was used to analyze the difference (p<0.05) of means across treatment groups. Results: There were no significant (p>0.05) changes in the body weight, hematological, biochemical and histology signs of toxicity for both male and female mice, except for the sodium level which was decreased in the mice treated with 2000 mg/kg of MECN (137±5.06) as compared to the 1000 (151±1.91) mg/kg as well as the control males (152±3.74) [F=4.87, p=0.03]. Conclusion: This study showed that MECN at dosages up to 2000 mg/kg is safe to be used in mice regardless of their sex. Overall, this study suggests the potential utility of MECN in the development of herbal drug formulations in vivo.
Authors and Affiliations
Samiaa Jamil Abdulwahid, Yong Meng Goh, Mahdi Ebrahimi, Norhafizah Mohtarrudin, Zailina Binti Hashim
Keywords
Related Articles
- EP ID EP566092
- DOI 10.22159/ijpps.2018v10i12.28075
- Views 71
- Downloads 0
PREBIOTIC INFLUENCE OF PLANTAGO OVATA ON FREE AND MICROENCAPSULATED L. CASEI–GROWTH KINETICS, ANTIMICROBIAL ACTIVITY AND MICROCAPSULES STABILITY
Objective: To study the enhancement of antimicrobial effects of probiotic Lactobacillus casei (L. casei) both in free and immobilized form and in the presence of a natural prebiotic-Psyllium seed husk.Methods: Arabinoxyl...
BIOCONTROL OF FUSARIUM OXYSPORUM IN UNSTERILIZED SOIL BY NOVEL STREPTOMYCES CACAOI SUBSP CACAOI [M20]
Objective: To find bio fungicide from mangrove actinomycetes for controlling seed and soil borne pathogen-Fusarium oxysporum. Methods: A total of 25 actinomycetes were isolated by pour plate method. These were screened f...
MEMECYLON SPECIES: A REVIEW OF TRADITIONAL INFORMATION AND TAXONOMIC DESCRIPTION
The present review is to avail the comprehensive information on taxonomy, phytochemistry and pharmacology of Indian Memecylon species. Memecylon is one of the complex genus of flowering plants and it is an important sour...
HYPOCHOLESTEROLEMIC EFFECT AND PIGMENTS COMPOSITION OF HERBAL MEDICINE CONTAINING HIGHER AND LOWER PLANTS
Objective: The objective of this research was to investigate the antihypercholesterolemic activity of herbal medicine which consisted of pigment-rich simplicia from higher and lower plants, as well as to determine its pi...
ENDOPHYTIC FUNGI FROM PHYLLANTHUS AMARUS SCHUM. & THONN. CAPABLE OF PRODUCING PHYLLANTHIN, HYPOPHYLLANTHIN AND/OR RELATED COMPOUNDS
Objective: To isolate endophytic fungi from various parts of Phyllanthus amarus (family Phyllanthaceae) and to probe for production of the bioactive compounds Phyllanthin and Hypophyllanthin.Methods: The fungi were isola...