Sulphated galactopyran derived from Gracilaria opuntia, a marine macroalgae restores the antioxidant metabolic enzymes during STZ induced diabetic rats
Journal Title: Journal of Coastal Life Medicine - Year 2017, Vol 5, Issue 2
Abstract
Objective: To screen the effect of sulphated galactopyran fraction isolated from Gracilaria opuntia (G. opuntia) (FM4) in streptozotocin (STZ) induced diabetic rats. Methods: In vitro antioxidant assays of FM4 were estimated by DPPH, ABTS, hydroxyl free radical and Nitric oxide free radical activities. FM4 was purified and characterized by 1H-NMR spectra and FTIR as sulphated galactopyran. Diabetes was induced intraperitonially by single dose of STZ (55 mg/kg body weight). FM4 was administrated orally (80, 100, 125 mg/kg BW) to diabetic rats for 60 days. The enzymatic and non-enzymatic antioxidants such as superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione-S-transferase (GST), lipid peroxidase (LPx), glutathione reduced (GSH), vitamin-C (VIT-C) and vitamin-E (VIT-E) levels were estimated. Glibenclamide was used as standard drug. Results: Our results demonstrated that the aqueous extract of G. opuntia possess free radical scavenging activity. During FM4 fraction treatment (100 mg/kg BW), the SOD, GPx, CAT, GST, GSH, VIT-C and VIT-E levels were significantly (P < 0.05) increased, and the LPx levels were decreased in different organs such as liver, kidney, brain and pancreas of diabetic rats. Conclusions: The sulphated galactopyran fraction of the marine macroalgae (G. opuntia) possesses the antioxidant activity which might help in the prevention of oxidative damage that occurs during diabetes.
First record of Necrobia rufipes (De Geer, 1775) (Coleoptera: Cleridae) from Khuzestan Province, southwest of Iran, a beetle species of forensic importance
Objective: To introduce Necrobia rufipes as a forensic important species in the study area. Methods: This study was performed using a laboratory bred rat (Wistar rat) weighing 352 g as a model for decomposition. The rat...
Study on enzymes of industrial interest in digestive viscera: Greater amberjack (Seriola dumerili)
Objective: To investigate the presence of industrial enzymes (trypsin and chymotrypsin) as well as collagenolytic and fibrinolytic activities of the neglected processing waste of greater amberjack Seriola dumerili (S. du...
Protective role of marine macroalgae extracts against STZ induced diabetic rats
Objective: To study the anti-diabetic activity of marine macroalgae extracts (n = 31), purification and characterization of sulphated galactopyran (SGP) from Gracilaria opuntia (FM4) in diabetic rats. Methods: The animal...
Occurrence of Cryptosporidium oocysts in edible frogs ( Rana species) sold for human consumption in Hanwa frog market, Zaria, Kaduna State, Nigeria
Objective: To determine the occurrence of Cryptosporidium oocysts in edible frogs (Rana spp.) sold at the Hanwa frog market, Zaria, Kaduna State, Nigeria. Methods: A total of 117 frogs (Rana spp.) were randomly collected...
In vivo hypoglycemic, antinociceptive and in vitro antioxidant activities of methanolic bark extract of Crataeva nurvala
Objective: To rationalize the folkloric use of hypoglycemic, antinociceptive and antioxidant potentials with phytochemical screening of methanolic bark extract of Crataeva nurvala (C. nurvala) in vivo and in vitro. Metho...