Suppression of Conjunctival Scarring by Chymase Inhibitor in a Canine Symblepharon Model
Journal Title: International Journal of Ophthalmology & Eye Science (IJOES) - Year 2016, Vol 0, Issue 6
Abstract
Purpose: To investigate the suppressive effects of a chymase inhibitor (CI) on conjunctival scarring in a canine model of symblepharon induced by alkali burns. Methods: Symblepharon models were made in beagle eyes. A cotton pad soaked in 1 N sodium hydroxide (NaOH) was placed in the conjunctival sac of each eye for 90 seconds, followed by washing the sac with 100 ml of physiological saline. Immediately post treatment, one eye drop (50 μl) including 1 mM-CI or the vehicle (CI-treated group and vehicle-treated group, respectively) was instilled in 5 eyes of 5 beagles once daily for 5 weeks. Fellow eyes were left untreated and served as the normal group. The severity of symblepharon was graded by macroscopic observation. After the eyes were enucleated, conjunctival and scleral tissue specimens were histologically evaluated. Results: Macroscopic observation revealed that symblepharon was induced in all NaOH-treated eyes, while symblepharon scores were significantly lower in the CI group than in the vehicle-treated group. Histological observation indicated a significant reduction in the adhered area in the CI-treated group compared to the vehicle-treated group. Immunohistochemical analysis demonstrated that vimentin-, α-smooth muscle actin-, chymase-, and angiotensin II-positive cells as well as mast cells increased in the vehicle-treated group compared to the normal group, whereas they were reduced in the CI-treated group. Conclusions: Our macroscopic and histological findings indicated that a multiple application of CI eye drops suppressed conjunctival scarring in a canine symblepharon model, suggesting that the topical application of CI may be a promising therapy for symblepharon.
Authors and Affiliations
Tetsuya Sugiyama
Keywords
Related Articles
- EP ID EP200098
- DOI 10.19070/2332-290X-SI07002
- Views 58
- Downloads 0
Exfoliation Syndrome
Exfoliation Syndrome
Lens Fluid Displacement and Accommodation
In this study, the biomechanics of the human lens geometry were numerically analyzed to quantitate micro-fluidic movement of lens fiber cells. Literature values were collected to develop a lens geometry paradigm related...
Orbital Lymphoma as Cause of Proptosis in Right Eye
Purpose: A case of B-cell non-Hodgkin lymphoma is presented in the right orbit, which, after treatment with excisional biopsy plus chemotherapy, reached a favorable resolution after 9 months of treatment. Case report: 83...
Vision Loss Case of Bardet Biedl Syndrome
We would like to report a rare case of Bardet Biedl Syndrome of a women forty-three years, known case of Retinitis pigmentosa presented for medical report, after ocular and systemic assessment we found she is obese, moon...
Transconjunctival Lower Lid Blepharoplasty Made Easy by Everting the Eyelid
Purpose: To evaluate the transconjunctival lower lid blepharoplasty using a Desmarres lid retractor to evert the eyelid. Design: Prospective study. Methods: Twenty-one cases of inferior blepharoplasty in 11 patients, per...