Suppression of nociception by Solanum incanum (Lin.) Diclomethane root extract is associated antiinflammatory activity
Journal Title: The Journal of Phytopharmacology - Year 2014, Vol 3, Issue 3
Abstract
Solanum incanum is an herb that is an important African folklore remedy for several ailments such as inflammation, pain, fever, microbial diseases, and neoplastic disorders. The herb possesses several pharmacological activities that include antinociceptive effect to thermal pain test models, antipyretic, antimicrobial and anticancer activity. However, there are no reported studies on its anti-inflammatory activity and effects on chemical pain test models. The aim of this study was to evaluate the effect of the root extract on inflammation and formalin pain test model. In the anti-inflammatory assay, white Wistar rats were injected intraperitoneally with doses of the herb diclofenac and the vehicle. Thirty minutes later the animals were injected with 50µg of 5% formalin in the sub-plantar region of the left hind paw to induce inflammation and the diameter of the paw measured using a digital caliper. The difference between the initial paw diameter and subsequent readings was quantified as the edema developed in the paw. To assess the effect of the herb on leukocyte migration, carrageenan was injected intraperitoneally into the white albino mice after thirty minutes following subcutaneous administration of the herb extracts and controls. Four hours later, normal saline was injected into the peritoneum and a peritoneal lavage performed and the total number of leukocytes in the fluid determined using a Neubauer chamber. In the antinociceptive assay, white Wistar rats were injected intraperitoneally with doses of the herb extracts. Thirty minutes later the animals were injected with 50µg of 5% formalin in the sub-plantar region of the left hind paw the total time spent in flinching, lifting, biting and licking the hind paw was quantified as the latency of nociception. The dichloromethane extract exhibited significant (p < 0.05) anti-inflammatory and 2rd phase antinociceptive effect, with 50 mg doses inhibiting highly significant (p < 0.001) effect in the early phase. Since the second phase of nociception is both inflammatory and neurogenic these results suggest that the anti-inflammatory activity of S. incanum plays an important role in its antinociception.
Authors and Affiliations
Mwonjoria J. K, Ngeranwa J. J. , Githinji C. G. , Kahiga T. , Kariuki H. N. , Waweru F. N
Keywords
Related Articles
- EP ID EP49558
- DOI -
- Views 209
- Downloads 0
Psychopharmacological studies of Mammea africana stem bark extract
Herbal medicine is an integral part of the health care system in most developed and developing countries of the world. Objective: The stem bark of Mammea africana used traditionally in the treatment of mental disorders...
Investigation of anti-arthritic activity (in-vitro models) of Hibiscus hispidissimus Griffith
Aim of the experiment: The present study was designed to investigate the anti-arthritic potential of the plant Hibiscus hispidissimus. Materials and Methods: The aerial parts of the plant was collected, dried and extra...
Anticonvulsant actions of ethanol stem bark extract of Trichilia roka (Meliaceae) in mice and chicks
The current antiseizure drugs present with side effects, some of which can lead to discontinuation of epilepsy pharmacotherapy, and a sizeable number of other drugs being less efficacious. Medicinal plants are used for...
Pharmacognostical and Phytochemical Evaluation of Pilla Shukra Nashaka Anjana Varti: An ocular Ayurvedic Formulations
Pilla Shukra Nashaka Anjana Varti is an important Ayurvedic formulation containing Haritaki (Terminalia chebula Retz.), Amalaki (Emblica officinalis Gaertn.), Bibhitaki (Terminalia bellerica Roxb.), Katuki (Picrohriza...
GC-MS analysis, antioxidant, antibacterial and anticancer activities of methanol bark extract of Albizia lebbeck (L.)
Albizia lebbeck is a deciduous tree with compound leaves, flat oblong fruits, round cream seeds are colored and wild growth. Albizia lebbeck is found throughout India, Bangladesh, tropical and subtropical Asia and Afri...