Synthesis and Characterization of Cisplatin-Loaded BSA (Bovine Serum Albumin) Nanoparticles as Drug Delivery System against Pancreatic Cancer Cells
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 9
Abstract
Cisplatin as chemotherapeutic agents is consideredone of important drug for treatment of many malignant disorders. Prolonged and Conventional administration methods for cisplatin reduce its effectiveness to the target organ beside side-effects, as nephrotoxicity and ototoxicity. Nanotechnology holds several promises in cancer therapeutics. It can increase the ability of the drug toward the target site of the tumor cells, reach otherwise lesser-accessible sites and reduce the frequency of administration. The present study explored the efficacy of cisplatin coated albumin nanoparticle as a sustained delivery system.To attain this goal, cisplatin was loaded onto albumin nanoparticles (NPs), characterization of the formed albumin nanoparticles was done using surface plasmon spectra using UV-spectroscopy.The morphology of nanoparticles (NPs) were determined by scanning electron microscope (SEM ), transmission electron microscope (TEM) and Fourier transform infrared spectroscopy (FTIR) was done. The effect of particles on RBCs was assayed.Cellular uptake of cisplatin loaded nano-albumin was qualitatively visualized by Confocal laser scanning microscopy.The anti-cancer efficacy of the cisplatin coated albumin nanoparticles were tested on pancreatic cancer cell lines by Dimethylthiazol Diphenyl Tetrazolium Bromide (MTT) assay. The result showed that nanoparticles have the ability for releasing the drug for more than 50 h,the release kinetics is diphasic.Transmission electron microscopy (TEM) investigated particle sizes in the range 55nm with encapsulation efficiency of 98%.Cisplatin-conjugated albumin nanoparticlesdid not exhibit any toxicity or hemolysis to the RBC‟s in hemolysis test assay.A significant decreasingin survival rate of the tumor cells withincreasing concentration of cisplatin loaded albumin nanoparticles. CLSM results showed cisplatin loaded albumin NPs could be absorbed easier and sooner into the nucleus than albuminNPs.Finally, it was found that cisplatin was an appropriate anticancer drug has the ability to be encapsulated in polymeric nanoparticle and released from the carriers with favor rate and no burst effect.
Authors and Affiliations
Sohier M. Syame, Zaki Monawar Eisa, Ragaa Eltayeb, Ashraf S. Hakim, MagdyKhalil M
Keywords
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