Synthesis, Characterization and Imaging of Fluorescine Isothiocyanate Conjugated Magnetite Nanoparticles in MCF 7 Breast Cancer Cell Lines
Journal Title: International Journal of Nanomaterials, Nanotechnology and Nanomedicine - Year 2017, Vol 3, Issue 2
Abstract
In this work we describe fabrication, characterization and possible application of FITC (fluorescine isothiocyanate) conjugated magnetite nanoparticles (MNPs) for biomedical applications such as imaging of cancer cells. The MNPs possessed octahedral-like geometry with almost completely dispersed distribution with high saturation magnetization. The final FMNPs (fluorescine isothiocyanate magnetite nanoparticles) absorption band showed 30 nm red shift towards longer wavelength compared to bare MNP and the laser-induced fluorescence was observed at 518 nm. The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) results showed that FITC conjugation diminishes the toxicity MNPs mainly due to the reduction of surface charge. Fluorescence microscopy confirmed the uptake and distribution of FMNPs in MCF 7 (Michigan Cancer Foundation-7) breast cancer cells, which suggests that it can be utilized for applications such as a magnetic fluorescent probe for bioassay.
Authors and Affiliations
Khosroshahi Mohammad E, Asemani Maral
Keywords
Related Articles
- EP ID EP335408
- DOI 10.17352/2455-3492.000020
- Views 48
- Downloads 0
Combined In vitro Effects of TiO2 Nanoparticles and Dimethyl Sulfoxide (DMSO) on HepG2 Hepatocytes
Introduction: Professional workers that manufacture or use titanium dioxide (TiO2)-based paints are exposed to potentially toxic TiO2 nanomaterials as well as to different paint solvents such as dimethyl sulfoxide (DMSO)...
Synthesis, Characterization and Antibacterial Activity of Ciprofloxacin Loaded Polymer Nanoparticles for Parenteral Application
Different polymerization techniques as particle formation processes for ciprofloxacin-loaded poly (butyl cyanoacrylate) nanoparticles (CfH-PBCN) were evaluated to choose the most appropriate in terms of the resulting nan...
Graphene Solar Cells-Will it be the Ultimate Power Converter?
Solar cells or photovoltaic (PV) cells involve the direct conversion of light energy into electrical energy. PV cells are basically p-n junctions made from layers of semiconducting materials. Under light illumination, ei...
Two Faces of Curcumin; A Molecular Nutrition and an Anti-Cancer Agent
Curcumin, a useful herbal medicine with anti-inflammatory and anti-cancer properties is insoluble in water which restricts its therapeutic properties; its prominent application in cancer treatment is limited due to curcu...
Self-Assembly as a Technique for Peptide-Based Materials
Molecular self-assembly is a key function in biology and has been developed as an elegant technique for fabrication of various complex structures and functional materials. Key importance for structural formation in terms...