SYNTHESIS OF N-4-METHOXYBENZOYL-N'-PHENYLUREA COMPOUND AND ACTIVITY TEST OF ANTICANCER AGAINST HeLa CELL LINE
Journal Title: World Journal of Pharmaceutical Research - Year 2018, Vol 7, Issue 4
Abstract
Research in the search for cancer drug compounds continues to be developed, considering that specific anticancer compound has not been obtained. Some of the urea derivative compounds are also being developed in the search for an anti-cancer compound which is potent with minimal side effects. Related to the explanation above, a urea derivative, which is N-phenylurea, would like to be developed. It would be reacted with 4-methoxybenzoyl chloride, thus N-4- methoxybenzoyl-N'-phenylurea compound would be obtained. The synthesis of N-4-methoxybenzoyl-N'-phenylurea was carried out by Schotten-Baumman method that had been modified, and then a test of purity was done by thin layer chromatography using 3 different kinds of eluent. The next phase was structure characterization which was carried out by using UV and IR spectrophotometry, then 1H-NMR spectrometry also MS, so that the structure from N-4- methoxybenzoyl-N'-phenylurea would be obtained. Anticancer activation test was conducted on HeLa cell line by using MTT assay, and the value of IC50 would be obtained. The compound that has been successfully synthesized is N-4-methoxybenzoyl-N'-phenylurea, with a yield of 51,23%. The purity test of N-4-methoxybenzoyl-N'-phenylurea was done by thin layer chromatography with 3 different types of eluent (hexane: ethyl acetate: methanol = 2: 3: 1; Hexane: acetone = 4: 2; Hexane: ethyl acetate = 4: 2) one single spot was obtained, which its value of Rf is different than the original compound, N-phenylurea. While the melting point of the compound is 158oC, thus it was seen that the compound has been formed and was different from the original compound, N-phenylurea. Anticancer activation test has been performed with MTT assay method by using HeLa cell line, the result obtained of IC50 is 6.50 mM more active than hydroxyurea is 170.57 mM as a standard. The conclusion is that N-4-methoxybenzoyl-N'phenylurea, have been successfully synthesized and has anticancer activity.
Authors and Affiliations
Bambang Tri Purwanto
Keywords
Related Articles
- EP ID EP620508
- DOI -
- Views 107
- Downloads 0
ASSESSMENT OF CINNAMON EFFECT TO REDUCE PRIMARY DYSMENORRHEA AND IMPROVE QUALITY OF LIFE AMONG FEMALES IN EASTERN REGION OF SAUDI ARABIA: A CROSS SECTIONAL SURVEY
Dysmenorrhea is a painful contraction in the uterus region happening before and/or during menstrual cycle menstrual cycle. It is a typical issue among adults females influencing around 40-80% of them begin a couple days...
IMPURITY PROFILING: OVERVIEW ON IMPURITY PROFILING AND REPORTING METHODOLOGIES ADOPTED BY UNITED STATES AND EUROPE
Impurities are not acceptable in drug formulation. It is considered as unwanted chemicals or organic material which remains with Active Pharmaceutical Ingredient (API’s). The impurity is produced either during formulatio...
ROLE OF LAGHUMALINIVASANT RASA IN UPVISHTAK (INTRA UTERINE GROWTH RETARDATION)
Intra uterine growth restriction (IUGR) refers to a condition in which a foetus is unable to achieve its genetically determined potential size. This functional definition seeks to identify a population of foetuses at ris...
“SEERAKA CHOORANAM” A SIDDHA FORMULATION FOR IRATHTHAK KOTHIPPU NOAI (HYPERTENSION)
Iraththak kothippu noai (Hypertension) is one of dangerous non communicable diseases in the world. National survey on health of Sri Lankans finds 18 percent of population suffers a chronic illness. Among the cases report...
A REVIEW ON AMARANTHUS TRICOLOR AS A TRADITIONAL MEDICINAL PLANT
The present article is based on Amaranthus tricolor belonging to family Amaranthacea. Amaranthus tricolor is a topical annual herb with long leaves and small black seed and it can attain a height of maximum 5fits. The st...