Temperature and Time Dependent Variations in Anti-Toxoplasma gondii Antibody Titrations in Serum Samples
Journal Title: Turkish Journal of Parasitology - Year 2021, Vol 45, Issue 1
Abstract
Objective: This study aimed to determine the temperature- and time-dependent variations in the anti-Toxoplasma gondii (T. gondii) antibody titres in serum samples collected from T. gondii-infected patients. Methods: During May 2017-February 2018, five serum samples (stored at different time periods and conditions) from pregnant or non-pregnant women aged ≥18 years who were infected with T. gondii and had applied to our Parasitology Department of Hafsa Sultan Hospital, Manisa Celal Bayar University, were investigated for the anti-T. gondii IgG antibody levels by enzyme linked fluorescent assay (ELFA). Results: The serum samples of five female volunteers who were infected with T. gondii that were stored at room temperature (20/25 °C), in a cargo package (+4/+8 °C), in a refrigerator (+4 °C), in a deep freezer (-16/-20 °C) and in an incubator (+37 °C) were tested at 0, 24, 48 and 72 hours after infection with the ELFA test. No statistically significant difference was observed in the anti-T. gondii IgG antibody titres (p>0.05). Conclusion: The results obtained from the patients infected with T. gondii at different times and conditions of up to 72 hours were not significantly affected clinically. Hence, more comprehensive data can be obtained by increasing the number of patients and storing the serum samples for more than 72 hours.
Authors and Affiliations
Emine Çoban, Ali Ahmet Kilimcioğlu
Keywords
Related Articles
- EP ID EP693308
- DOI 10.4274/tpd.galenos.2020.7074
- Views 67
- Downloads 0
Toxocariasis Presenting with Hypereosinophilia and Lymphadenopathy: A Case Report
Human toxocariasis is a parasitic disease transmitted to humans by ingestion of Toxocara spp. larvae. Rural area residents and children have higher risk for this parasitic infection. The disease is frequently asymptomati...
A Case of Rheumatoid Arthritis with Strongyloides stercoralis Hyperinfection
The patient was a 83-year- old male who worked as a farmer. He had complaints of weight loss, abdominal pain and joint pains for almost 5 months. Twenty days ago, the patient was checked at another hospital for complaint...
Comparative Evaluation of the Efficacy of Indirect Hemagglutination Test and Radiological Methods in the Pre-diagnosis of Cystic Echinococcosis
Objective: Cystic echinococcosis (CE) is a common public health concern in Turkey. In this study, we investigated the agreement between the results of radiological imaging methods and serological tests through a 5-year r...
The Retrospective Analysis of Demodex spp. Results in Aydın Adnan Menderes University Faculty of Medicine Hospital Parasitology Laboratory
Objective: Demodex spp. is one of the most common ectoparasites in humans. The aim of the present study is to evaluate the positivity of Demodex spp. in our Parasitology Laboratory, retrospectively. Methods: The study i...
Infestation of Chirodiscoides caviae in a Guinea pig (Cavia porcellus) at Turkey and Fipronil Treatment
Kırıkkale University Faculty of Veterinary Medicine Parasitology Department Laboratory has brought a male kobay for 3-4 months with the complaint having a large number of black mites on his hair by a student of the same...